Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells
Joint Authors
Taniuchi, Fumiko
Higai, Koji
Tanaka, Tomomi
Azuma, Yutaro
Matsumoto, Kojiro
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The α1,2-fucosyltransferase I (FUT1) enzyme is important for the biosynthesis of H antigens, Lewis B, and Lewis Y.
In this study, we clarified the transcriptional regulation of FUT1 in the DLD-1 colon cancer cell line, which has high expression of Lewis B and Lewis Y antigens, expresses the FUT1 gene, and shows α1,2-fucosyltransferase (FUT) activity.
5′-rapid amplification of cDNA ends revealed a FUT1 transcriptional start site −10 nucleotides upstream of the site registered at NM_000148 in the DataBase of Human Transcription Start Sites (DBTSS).
Using the dual luciferase assay, FUT1 gene expression was shown to be regulated at the region −91 to −81 nt to the transcriptional start site, which contains the Elk-1 binding site.
Site-directed mutagenesis of this region revealed the Elk-1 binding site to be essential for FUT1 transcription.
Furthermore, transfection of the dominant negative Elk-1 gene, and the chromatin immunoprecipitation (CHIp) assay, supported Elk-1-dependent transcriptional regulation of FUT1 gene expression in DLD-1 cells.
These results suggest that a defined region in the 5′-flanking region of FUT1 is critical for FUT1 transcription and that constitutive gene expression of FUT1 is regulated by Elk-1 in DLD-1 cells.
American Psychological Association (APA)
Taniuchi, Fumiko& Higai, Koji& Tanaka, Tomomi& Azuma, Yutaro& Matsumoto, Kojiro. 2013. Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1011477
Modern Language Association (MLA)
Taniuchi, Fumiko…[et al.]. Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells. The Scientific World Journal No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1011477
American Medical Association (AMA)
Taniuchi, Fumiko& Higai, Koji& Tanaka, Tomomi& Azuma, Yutaro& Matsumoto, Kojiro. Transcriptional Regulation of Fucosyltransferase 1 Gene Expression in Colon Cancer Cells. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1011477
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1011477