Cationic Polymers Inhibit the Conductance of Lysenin Channels
Joint Authors
Fologea, Daniel
Krueger, Eric
Rossland, Steve
Bryant, Sheenah
Foss, Wylie
Clark, Tyler
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The pore-forming toxin lysenin self-assembles large and stable conductance channels in natural and artificial lipid membranes.
The lysenin channels exhibit unique regulation capabilities, which open unexplored possibilities to control the transport of ions and molecules through artificial and natural lipid membranes.
Our investigations demonstrate that the positively charged polymers polyethyleneimine and chitosan inhibit the conducting properties of lysenin channels inserted into planar lipid membranes.
The preservation of the inhibitory effect following addition of charged polymers on either side of the supporting membrane suggests the presence of multiple binding sites within the channel's structure and a multistep inhibition mechanism that involves binding and trapping.
Complete blockage of the binding sites with divalent cations prevents further inhibition in conductance induced by the addition of cationic polymers and supports the hypothesis that the binding sites are identical for both multivalent metal cations and charged polymers.
The investigation at the single-channel level has shown distinct complete blockages of each of the inserted channels.
These findings reveal key structural characteristics which may provide insight into lysenin’s functionality while opening innovative approaches for the development of applications such as transient cell permeabilization and advanced drug delivery systems.
American Psychological Association (APA)
Fologea, Daniel& Krueger, Eric& Rossland, Steve& Bryant, Sheenah& Foss, Wylie& Clark, Tyler. 2013. Cationic Polymers Inhibit the Conductance of Lysenin Channels. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1011959
Modern Language Association (MLA)
Fologea, Daniel…[et al.]. Cationic Polymers Inhibit the Conductance of Lysenin Channels. The Scientific World Journal No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1011959
American Medical Association (AMA)
Fologea, Daniel& Krueger, Eric& Rossland, Steve& Bryant, Sheenah& Foss, Wylie& Clark, Tyler. Cationic Polymers Inhibit the Conductance of Lysenin Channels. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1011959
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1011959