Basic and lectin histochemistry of the pregnant and non pregnant vaginal mucosa of she-camel

Abstract EN

Traditional non-lection staining methods as periodic acid Schiff (PAS) and Alcan blue at pH 2.5 (Ab 2.5), as well as horseradish peroxidase (HRP) conjugated Helix pomatum agglutinin (HPA) and Girffonia simplicifolia agglutinin (GSA-1) were used to investigate the pregnancy associated changes on the vaginal mucosa of She-camel.

There was remarkable increase in the glycoproteins (GPs) with oxidizable vicinal doles in the upper most layer of the vaginal epithelium of the pregnant She-camel.

At the same time, only little increase in the GPs with carboxyl group and / or with O-sulfate ester was limited to the goblet cells of the vaginal mucosa.

Significant changes could be detected in the lection histochemistry in pregnant She-camel comparing with the non-pregnant one.

Abundant amount of N-acetylgalactosamine detected in the plasmalemma of all layers of the vaginal mucosa of She-camel especially in the mucous secreting cells.

The changes of D-galactic and / or N- acetylgalactosamine were restricted to the goblet cells.