![](/images/graphics-bg.png)
Investigation of Biofilm Forming Ability in Staphylococci Causing Bovine Mastitis Using Phenotypic and Genotypic Assays
Joint Authors
Asfour, Hanaa A. E.
Darwish, Samah Fikri
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
A total of 40 S.
aureus and 68 coagulase negative Staphylococcus (CNS) isolates from bovine subclinical mastitis were investigated for their ability to form biofilm as one of the most important virulence factors.Using Congo Red Agar (CRA) method, 32.5%, 35%, and 32.5% of S.
aureus strains were strong, intermediate, and negative biofilm producers, while in CNS the percentages were 29.5%, 42.6%, and 27.9%, respectively.
By microtiter plate (MTP) method, 52.5%, 27.5%, and 20% of S.
aureus isolates were strong, moderate, and weak biofilm producers, while in CNS the percentages were 44%, 30.9%, and 19.2%, respectively.
Indian ink staining was used to detect the EPS layer of biofilm producers.
All isolates were screened for presence of biofilm related genes, eno, icaA, icaD, and bap.
In S.
aureus isolates, the positive rates of eno, icaA, icaD, and bap genes were 75%, 15%, 62.5%, and 2.5% while in CNS were 92.6%, 5.9%, 47.1%, and 4.4%, respectively.
The eno gene had the highest rate while the bap gene had the lowest rate.
Presence of icaA and icaD genes was not always correlated with biofilm production.
This study demonstrated high prevalence of Staphylococcus biofilm producers among bovine mastitis in Egypt.
Therefore, attention must be paid toward implementation of new ways for effective treatment of such infections.
American Psychological Association (APA)
Darwish, Samah Fikri& Asfour, Hanaa A. E.. 2013. Investigation of Biofilm Forming Ability in Staphylococci Causing Bovine Mastitis Using Phenotypic and Genotypic Assays. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1012094
Modern Language Association (MLA)
Darwish, Samah Fikri& Asfour, Hanaa A. E.. Investigation of Biofilm Forming Ability in Staphylococci Causing Bovine Mastitis Using Phenotypic and Genotypic Assays. The Scientific World Journal No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1012094
American Medical Association (AMA)
Darwish, Samah Fikri& Asfour, Hanaa A. E.. Investigation of Biofilm Forming Ability in Staphylococci Causing Bovine Mastitis Using Phenotypic and Genotypic Assays. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1012094
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1012094