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Effect of Vasoactive Intestinal Peptide (VIP) on NKG2D Signal Pathway and Its Contribution to Immune Escape of MKN45 Cells
Joint Authors
Li, Guohua
Wang, Chong
Zhou, Xi-Jin
Li, Yuan-yuan
Wan, Juan
Yang, Le-ying
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
To investigate VIP effect on the cytotoxicity of NK cell to gastric cancer cells in vitro and the relation between the effect with the NKG2D signal molecules in NK cells.
Material and Methods.
NK cells were purified from peripheral blood mononuclear cells (PBMC).
Before and after NK cells were incubated with VIP or its antagonist (D-p-Cl-Phe6,Leu17)-VIP, we detected the cytotoxicity of NK cells to MKN45 gastric cancer cells by MTT and detected the expressions of NKG2D, DAP10, and NF-κB proteins and mRNAs in NK cells by immunocytochemistry and RT-PCR in those conditions.
Then we analyzed the effect of VIP and its antagonist on the cytotocicity of NK cell to gastric cancer cells and on expressions of NKG2D, DAP10, and NF-κB signal molecules in NK cells.
Results.
VIP could inhibit the cytotoxicity of NK cells to MKN45 cells and could inhibit the expressions of NKG2D, DAP10, and NF-κB in NK cells.
However, (D-p-Cl-Phe6, Leu17)-VIP could reverse those effects.
Conclusions.
The VIP inhibited the cytotoxicity of NK cell to MKN45 cells which might get through inhibiting the expressions of NKG2D signal molecules in NK cells.
This may be one mechanism of gastric cancer cells escaping organism immune clearance.
American Psychological Association (APA)
Wang, Chong& Zhou, Xi-Jin& Li, Yuan-yuan& Wan, Juan& Yang, Le-ying& Li, Guohua. 2013. Effect of Vasoactive Intestinal Peptide (VIP) on NKG2D Signal Pathway and Its Contribution to Immune Escape of MKN45 Cells. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1012283
Modern Language Association (MLA)
Wang, Chong…[et al.]. Effect of Vasoactive Intestinal Peptide (VIP) on NKG2D Signal Pathway and Its Contribution to Immune Escape of MKN45 Cells. The Scientific World Journal No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1012283
American Medical Association (AMA)
Wang, Chong& Zhou, Xi-Jin& Li, Yuan-yuan& Wan, Juan& Yang, Le-ying& Li, Guohua. Effect of Vasoactive Intestinal Peptide (VIP) on NKG2D Signal Pathway and Its Contribution to Immune Escape of MKN45 Cells. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1012283
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1012283