Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9
Joint Authors
Chellaiah, Meenakshi A.
Gupta, Aditi
Cao, Wei
Sadashivaiah, Kavitha
Chen, Wantao
Schneider, Abraham
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Cell surface interaction of CD44 and MMP9 increases migration and invasion of PC3 cells.
We show here that stable knockdown of MMP9 in PC3 cells switches CD44 isoform expression from CD44s to CD44v6 which is more glycosylated.
These cells showed highly adhesive morphology with extensive cell spreading which is due to the formation of focal adhesions and well organized actin-stress fibers.
MMP9 knockdown blocks invadopodia formation and matrix degradation activity as well.
However, CD44 knockdown PC3 cells failed to develop focal adhesions and stress fibers; hence these cells make unstable adhesions.
A part of the reason for these changes could be caused by silencing of CD44v6 as well.
Immunostaining of prostate tissue microarray sections illustrated significantly lower levels of CD44v6 in adenocarcinoma than normal tissue.
Our results suggest that interaction between CD44 and MMP9 is a potential mechanism of invadopodia formation.
CD44v6 expression may be essential for the protection of non-invasive cellular phenotype.
CD44v6 decrease may be a potential marker for prognosis and therapeutics.
American Psychological Association (APA)
Gupta, Aditi& Cao, Wei& Sadashivaiah, Kavitha& Chen, Wantao& Schneider, Abraham& Chellaiah, Meenakshi A.. 2013. Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1012418
Modern Language Association (MLA)
Gupta, Aditi…[et al.]. Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9. The Scientific World Journal No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-1012418
American Medical Association (AMA)
Gupta, Aditi& Cao, Wei& Sadashivaiah, Kavitha& Chen, Wantao& Schneider, Abraham& Chellaiah, Meenakshi A.. Promising Noninvasive Cellular Phenotype in Prostate Cancer Cells Knockdown of Matrix Metalloproteinase 9. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-1012418
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1012418