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Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase
Joint Authors
Tayyab, Saad
Abdul Kadir, Habsah
Zaroog, Mohammed Suleiman
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Different spectral probes were employed to study the stabilizing effect of various polyols, such as, ethylene glycol (EG), glycerol (GLY), glucose (GLC) and trehalose (TRE) on the native (N), the acid-denatured (AD) and the thermal-denatured (TD) states of Aspergillus niger glucoamylase (GA).
Polyols induced both secondary and tertiary structural changes in the AD state of enzyme as reflected from altered circular dichroism (CD), tryptophan (Trp), and 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence characteristics.
Thermodynamic analysis of the thermal denaturation curve of native GA suggested significant increase in enzyme stability in the presence of GLC, TRE, and GLY (in decreasing order) while EG destabilized it.
Furthermore, CD and fluorescence characteristics of the TD state at 71°C in the presence of polyols showed greater effectiveness of both GLC and TRE in inducing native-like secondary and tertiary structures compared to GLY and EG.
American Psychological Association (APA)
Zaroog, Mohammed Suleiman& Abdul Kadir, Habsah& Tayyab, Saad. 2013. Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1012532
Modern Language Association (MLA)
Zaroog, Mohammed Suleiman…[et al.]. Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase. The Scientific World Journal No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1012532
American Medical Association (AMA)
Zaroog, Mohammed Suleiman& Abdul Kadir, Habsah& Tayyab, Saad. Stabilizing Effect of Various Polyols on the Native and the Denatured States of Glucoamylase. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1012532
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1012532