Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues
Joint Authors
Chen, Yun
Tsai, Ya-Hui
Liu, Yuan-An
Lee, Shih-Hua
Tseng, Sheng-Hong
Tang, Shiue-Cheng
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Two-dimensional (2D) histopathology is the standard analytical method for intestinal biopsied tissues; however, the role of 3-dimensional (3D) imaging system in the analysis of the intestinal tissues is unclear.
The 3D structure of the crypt organoids from the intestinal stem cell culture and intestinal tissues from the donors and recipients after intestinal transplantation was observed using a 3D imaging system and compared with 2D histopathology and immunohistochemistry.
The crypt organoids and intestinal tissues showed well-defined 3D structures.
The 3D images of the intestinal tissues with acute rejection revealed absence of villi and few crypts, which were consistent with the histopathological features.
In the intestinal transplant for megacystis microcolon intestinal hypoperistalsis syndrome, the donor’s intestinal tissues had well-developed nerve networks and interstitial cells of Cajal (ICCs) in the muscle layer, while the recipient’s intestinal tissues had distorted nerve network and the ICCs were few and sparsely distributed, relative to those of the donor.
The 3D images showed a clear spatial relationship between the microstructures of the small bowel and the features of graft rejection.
In conclusion, integration of the 3D imaging and 2D histopathology provided a global view of the intestinal tissues from the transplant patients.
American Psychological Association (APA)
Chen, Yun& Tsai, Ya-Hui& Liu, Yuan-An& Lee, Shih-Hua& Tseng, Sheng-Hong& Tang, Shiue-Cheng. 2013. Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1012628
Modern Language Association (MLA)
Chen, Yun…[et al.]. Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues. The Scientific World Journal No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-1012628
American Medical Association (AMA)
Chen, Yun& Tsai, Ya-Hui& Liu, Yuan-An& Lee, Shih-Hua& Tseng, Sheng-Hong& Tang, Shiue-Cheng. Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-1012628
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1012628