![](/images/graphics-bg.png)
The Determination of Six Ionophore Coccidiostats in Feed by Liquid Chromatography with Postcolumn Derivatisation and SpectrofotometricFluorescence Detection
Joint Authors
Olejnik, Małgorzata
Jedziniak, Piotr
Szprengier-Juszkiewicz, Teresa
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The control of levels of anticoccidial feed additives in targeted feeds plays an important role in the assurance of efficiency of animal treatment, prevention of drug resistance, and food safety.
The robust and labour-efficient method for the simultaneous determination of six ionophore coccidiostats (lasalocid, maduramicin, monensin, narasin, salinomycin, and semduramicin) in targeted feed has been developed.
Properly grinded and homogenized feed sample was spiked with internal standard (monesin methyl ester) and extracted with methanol.
The extract was analysed with reversed phase HPLC without any further purification.
The separation of the analytes with conventional C18 and core-shell columns was compared.
Lasalocid was analysed with fluorescence detection, whereas other ionophores were detected with UV-Vis detector after derivatisation with vanillin in the presence of sulfuric acid.
Fortified samples and targeted feeds at authorized levels were used for method validation.
Recovery was in the range of 85–110%, depending on the analyte.
The within-laboratory reproducibility did not exceed the target value from Horwitz equation.
The results of the proficiency tests (z-scores in the range of −1.0 to 1.9) confirmed the reliability of the developed protocol.
American Psychological Association (APA)
Olejnik, Małgorzata& Jedziniak, Piotr& Szprengier-Juszkiewicz, Teresa. 2013. The Determination of Six Ionophore Coccidiostats in Feed by Liquid Chromatography with Postcolumn Derivatisation and SpectrofotometricFluorescence Detection. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1012849
Modern Language Association (MLA)
Olejnik, Małgorzata…[et al.]. The Determination of Six Ionophore Coccidiostats in Feed by Liquid Chromatography with Postcolumn Derivatisation and SpectrofotometricFluorescence Detection. The Scientific World Journal No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-1012849
American Medical Association (AMA)
Olejnik, Małgorzata& Jedziniak, Piotr& Szprengier-Juszkiewicz, Teresa. The Determination of Six Ionophore Coccidiostats in Feed by Liquid Chromatography with Postcolumn Derivatisation and SpectrofotometricFluorescence Detection. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-1012849
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1012849