Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy
Joint Authors
Liu, Chao
Wang, Xinwei
Zhou, Yan
Liu, Yuliang
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-23
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research.
Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity, fluorophore concentration, and photobleaching compared to fluorescence intensity imaging techniques.
Time-gated fluorescence lifetime imaging microscopy (FLIM) can provide high resolution and high imaging frame during mature FLIM methods.
An abstract time-gated FLIM model was given, and important temporal parameters are shown as well.
Aiming at different applications of steady and transient fluorescence processes, two different operation modes, timing and lifetime computing algorithm are designed.
High resolution and high frame can be achieved by one-excitation one-sampling mode and least square algorithm for steady imaging applications.
Correspondingly, one-excitation two-sampling mode and rapid lifetime determination algorithm contribute to transient fluorescence situations.
American Psychological Association (APA)
Liu, Chao& Wang, Xinwei& Zhou, Yan& Liu, Yuliang. 2013. Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1012907
Modern Language Association (MLA)
Liu, Chao…[et al.]. Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy. The Scientific World Journal No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1012907
American Medical Association (AMA)
Liu, Chao& Wang, Xinwei& Zhou, Yan& Liu, Yuliang. Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1012907
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1012907