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In Vitro Activation and Inhibition of Recombinant EGFR Tyrosine Kinase Expressed in Escherichia coli
Joint Authors
Elloumi-Mseddi, Jihene
Jellali, Karim
Aifa, Sami
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-25
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The present work concerns the heterologous expression of the intracellular domain harbouring the tyrosine kinase activity of the epidermal growth factor receptor (EGFR).
Protein expression was improved thanks to the deletion of a 13-amino acid peptide of the juxtamembrane region (JM).
The recombinant proteins were produced as a glutathione S-transferase (GST) fusion in Escherichia coli, and the solubilisation was performed by sarkosyl addition during extraction.
The produced proteins spontaneously dimerize allowing the activation of the tyrosine kinase domain in the presence of [γ-32P]ATP.
The activity assay has revealed the autophosphorylation of EGFR proteins which was decreased in the presence of genistein.
Our system could facilitate the screening of EGFR inhibitors without the need of adding an exogenous substrate.
American Psychological Association (APA)
Elloumi-Mseddi, Jihene& Jellali, Karim& Aifa, Sami. 2013. In Vitro Activation and Inhibition of Recombinant EGFR Tyrosine Kinase Expressed in Escherichia coli. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1012915
Modern Language Association (MLA)
Elloumi-Mseddi, Jihene…[et al.]. In Vitro Activation and Inhibition of Recombinant EGFR Tyrosine Kinase Expressed in Escherichia coli. The Scientific World Journal No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-1012915
American Medical Association (AMA)
Elloumi-Mseddi, Jihene& Jellali, Karim& Aifa, Sami. In Vitro Activation and Inhibition of Recombinant EGFR Tyrosine Kinase Expressed in Escherichia coli. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-1012915
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1012915