Optimization of Extraction Parameters by Using Response Surface Methodology, Purification, and Identification of Anthocyanin Pigments in Melastoma malabathricum Fruit
Joint Authors
Taha, Rosna Mat
Anuar, Nordiyanah
Mohd Adnan, Ahmad Faris
Saat, Naziz
Aziz, Norkasmani
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Anthocyanins not just have various benefits in food industry but also have been used as natural colourants in cosmetic, coating products and as potential natural photosensitizers in solar cell.
Thus, the main purpose of this study was to obtain information on the maximum yield of anthocyanin that can be recovered from Melastoma malabathricum fruit.
Factors such as extraction temperature, extraction time, and solid to liquid ratio were identified to be significantly affecting anthocyanin extraction efficiency.
By using three-level three-factor Box-Behnken design, the optimized conditions for anthocyanin extraction by acidified methanol (R2=0.972) were temperature of 60°C, time of 86.82 min, and 0.5 : 35 (g/mL) solid to liquid ratio while the optimum extraction conditions by acidified ethanol (R2=0.954) were temperature of 60°C, time of 120 min, and 0.5 : 23.06 (g/mL) solid to liquid ratio.
The crude anthocyanin extract was further purified by using Amberlite XAD-7 and Sephadex LH-20 column chromatography.
Identification of anthocyanins revealed the presence of cyanidin dihexoside, cyanidin hexoside, and delphinidin hexoside as the main anthocyanins in M.
malabathricum fruit.
American Psychological Association (APA)
Anuar, Nordiyanah& Mohd Adnan, Ahmad Faris& Saat, Naziz& Aziz, Norkasmani& Taha, Rosna Mat. 2013. Optimization of Extraction Parameters by Using Response Surface Methodology, Purification, and Identification of Anthocyanin Pigments in Melastoma malabathricum Fruit. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1012924
Modern Language Association (MLA)
Anuar, Nordiyanah…[et al.]. Optimization of Extraction Parameters by Using Response Surface Methodology, Purification, and Identification of Anthocyanin Pigments in Melastoma malabathricum Fruit. The Scientific World Journal No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1012924
American Medical Association (AMA)
Anuar, Nordiyanah& Mohd Adnan, Ahmad Faris& Saat, Naziz& Aziz, Norkasmani& Taha, Rosna Mat. Optimization of Extraction Parameters by Using Response Surface Methodology, Purification, and Identification of Anthocyanin Pigments in Melastoma malabathricum Fruit. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1012924
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1012924