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Agrobacterium tumefaciens-Mediated Transformation of Valsa mali: An Efficient Tool for Random Insertion Mutagenesis
Joint Authors
Wang, Caixia
Guan, Xiangnan
Wang, Hanyan
Li, Guifang
Dong, Xiangli
Wang, Guoping
Li, Baohua
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Valsa mali is a causal agent of apple and pear trees canker disease, which is a destructive disease that causes serious economic losses in eastern Asia, especially in China.
The lack of an efficient transformation system for Valsa mali retards its investigation, which poses difficulties to control the disease.
In this research, a transformation system for this pathogen was established for the first time using A.
tumefaciens-mediated transformation (ATMT), with the optimal transformation conditions as follows: 106/mL conidia suspension, cocultivation temperature 22°C, cocultivation time 72 hours, and 200 μM acetosyringone (AS) in the inductive medium.
The average transformation efficiency was 1015.00 ± 37.35 transformants per 106 recipient conidia.
Thirty transformants were randomly selected for further confirmation and the results showed the presence of T-DNA in all hygromycin B resistant transformants and also revealed random and single gene integration with genetic stability.
Compared with wild-type strain, those transformants exhibited various differences in morphology, conidia production, and conidia germination ability.
In addition, pathogenicity assays revealed that 14 transformants had mitigated pathogenicity, while one had enhanced infection ability.
The results suggest that ATMT of V.
mali is a useful tool to gain novel insight into this economically important pathogen at molecular levels.
American Psychological Association (APA)
Wang, Caixia& Guan, Xiangnan& Wang, Hanyan& Li, Guifang& Dong, Xiangli& Wang, Guoping…[et al.]. 2013. Agrobacterium tumefaciens-Mediated Transformation of Valsa mali: An Efficient Tool for Random Insertion Mutagenesis. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1013246
Modern Language Association (MLA)
Wang, Caixia…[et al.]. Agrobacterium tumefaciens-Mediated Transformation of Valsa mali: An Efficient Tool for Random Insertion Mutagenesis. The Scientific World Journal No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-1013246
American Medical Association (AMA)
Wang, Caixia& Guan, Xiangnan& Wang, Hanyan& Li, Guifang& Dong, Xiangli& Wang, Guoping…[et al.]. Agrobacterium tumefaciens-Mediated Transformation of Valsa mali: An Efficient Tool for Random Insertion Mutagenesis. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-1013246
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1013246