Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis
Joint Authors
Díaz Lantada, Andrés
Alarcón Iniesta, Hernán
Pareja Sánchez, Beatriz
García-Ruíz, Josefa Predestinación
Source
Advances in Materials Science and Engineering
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-02
Country of Publication
Egypt
No. of Pages
10
Abstract EN
In this study, we present a promising approach for the rapid development of porous polydimethylsiloxane (PDMS) scaffold prototypes, with outer geometry defined from the design stage, according to the form of conventional implants or adapted to patients’ biostructures.
The manufacture method is based on phase separation processes using materials obtained by casting within additive rapid prototyped molds.
We include a comparative study of PDMS sponges obtained by different simple processes.
Final in vitro assessment is carried out using hMSCs (bone marrow-derived human mesenchymal stem cells), cultured onto porous PDMS scaffolds functionalized with aminopropyltriethoxysilane (APTS) and equilibrated with a trophic factors medium produced by the cells.
Results show that porous PDMS scaffold prototypes are excellent 3D platforms for hMSCs adhesion.
Furthermore, this PDMS-3D niche, seeded with hMSCs and chondrogenic incubation medium during three weeks, showed a successful chondrogenesis determined by collagen type II expression.
Thus, results show a versatile method to produce a 3D niche to address questions about cartilage and endochondral bone formation or skeleton tissues clinical approaches.
American Psychological Association (APA)
Díaz Lantada, Andrés& Alarcón Iniesta, Hernán& Pareja Sánchez, Beatriz& García-Ruíz, Josefa Predestinación. 2014. Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis. Advances in Materials Science and Engineering،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1015752
Modern Language Association (MLA)
Díaz Lantada, Andrés…[et al.]. Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis. Advances in Materials Science and Engineering No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1015752
American Medical Association (AMA)
Díaz Lantada, Andrés& Alarcón Iniesta, Hernán& Pareja Sánchez, Beatriz& García-Ruíz, Josefa Predestinación. Free-Form Rapid Prototyped Porous PDMS Scaffolds Incorporating Growth Factors Promote Chondrogenesis. Advances in Materials Science and Engineering. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1015752
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1015752