Emodin Augments Cisplatin Cytotoxicity in Platinum-Resistant Ovarian Cancer Cells via ROS-Dependent MRP1 Downregulation
Joint Authors
Ma, Jun
Yang, Jie
Wang, Chao
Zhang, Nan
Dong, Ying
Wang, Chengjie
Wang, Yu
Lin, Xinjian
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The intracellular level of reactive oxygen species (ROS) is closely associated with chemosensitivity of cancer cells.
Overexpression of ATP binding cassette transporter MRP1 is correlated with resistance to platinum drugs.
In this study, we tested the hypothesis that emodin, a potent ROS generator, may increase sensitivity of cisplatin-(cDDP-) resistant ovarian carcinoma cells to cDDP cytotoxicity via ROS-mediated suppression of MRP1 expression.
Using the isogenic pair of the human ovarian carcinoma cell line COC1 and its cDDP resistant variant COC1/DDP, we found that ROS level in the cDDP-sensitive ovarian cancer cells was significantly higher than that in the cDDP-resistant cells.
Emodin enhanced ROS production in COC1/DDP cells and consequently sensitized them to cDDP-induced apoptosis.
These effects were reversed by addition of the antioxidant N-acetyl-L-cysteine (NAC).
Cotreatment with emodin and cDDP inhibited the tumor growth in vivo by increasing tumor cell apoptosis.
The emodin-enhanced cDDP cytotoxicity was attributable to downregulation of multidrug resistance-related protein 1 (MRP1) expression.
Together, these results suggest that emodin could act as an adjunct to enhance the anticancer effect of cDDP likely through ROS-related downregulation of MRP1 expression, and may be of therapeutic potential in cDDP-refractory ovarian carcinomas.
American Psychological Association (APA)
Ma, Jun& Yang, Jie& Wang, Chao& Zhang, Nan& Dong, Ying& Wang, Chengjie…[et al.]. 2014. Emodin Augments Cisplatin Cytotoxicity in Platinum-Resistant Ovarian Cancer Cells via ROS-Dependent MRP1 Downregulation. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016090
Modern Language Association (MLA)
Ma, Jun…[et al.]. Emodin Augments Cisplatin Cytotoxicity in Platinum-Resistant Ovarian Cancer Cells via ROS-Dependent MRP1 Downregulation. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1016090
American Medical Association (AMA)
Ma, Jun& Yang, Jie& Wang, Chao& Zhang, Nan& Dong, Ying& Wang, Chengjie…[et al.]. Emodin Augments Cisplatin Cytotoxicity in Platinum-Resistant Ovarian Cancer Cells via ROS-Dependent MRP1 Downregulation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016090
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016090