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Interaction Analysis through Proteomic Phage Display
Joint Authors
Sundell, Gustav N.
Ivarsson, Ylva
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Phage display is a powerful technique for profiling specificities of peptide binding domains.
The method is suited for the identification of high-affinity ligands with inhibitor potential when using highly diverse combinatorial peptide phage libraries.
Such experiments further provide consensus motifs for genome-wide scanning of ligands of potential biological relevance.
A complementary but considerably less explored approach is to display expression products of genomic DNA, cDNA, open reading frames (ORFs), or oligonucleotide libraries designed to encode defined regions of a target proteome on phage particles.
One of the main applications of such proteomic libraries has been the elucidation of antibody epitopes.
This review is focused on the use of proteomic phage display to uncover protein-protein interactions of potential relevance for cellular function.
The method is particularly suited for the discovery of interactions between peptide binding domains and their targets.
We discuss the largely unexplored potential of this method in the discovery of domain-motif interactions of potential biological relevance.
American Psychological Association (APA)
Sundell, Gustav N.& Ivarsson, Ylva. 2014. Interaction Analysis through Proteomic Phage Display. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1016123
Modern Language Association (MLA)
Sundell, Gustav N.& Ivarsson, Ylva. Interaction Analysis through Proteomic Phage Display. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1016123
American Medical Association (AMA)
Sundell, Gustav N.& Ivarsson, Ylva. Interaction Analysis through Proteomic Phage Display. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1016123
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016123