Epinephrine Enhances the Response of Macrophages under LPS Stimulation
Joint Authors
Zhou, Jianyun
Yan, Jun
Liang, Huaping
Jiang, Jianxin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Trauma associated with infection may directly trigger a neuroendocrine reaction in vivo while the hormone epinephrine is known to mediate immune responses to inflammation after injury.
However, the role of epinephrine during the earliest stage of trauma still remains unclear.
We therefore explored the role of epinephrine on activated macrophages under LPS stimulation in vitro as well as the mechanisms underlying its effect.
Dose- and time-dependent effects of epinephrine on macrophage immune function were assessed after LPS activation.
We also employed CD14 siRNA interference to investigate whether CD14 played a role in the mechanism underlying the effect of epinephrine on LPS-induced macrophage responses.
Our results showed that epinephrine pretreatment (10 ng/mL) significantly promoted immune responses from LPS stimulated macrophages, including phagocytic rate, phagocytic index, TNFα/IL-1β/IL-10 secretion, and CD14 expression (P < 0.05).
Moreover, TNFα/IL-1β/IL-10 levels attained their peak value 1 hour after incubation with 10 ng/mL epinephrine (P < 0.05), and CD14 siRNA transfection dramatically decreased phagocytosis and cytokine secretion by LPS-activated macrophages (P < 0.05).
We therefore conclude that 10 ng/mL epinephrine enhances immune responses from macrophages under LPS stimulation and that the underlying mechanism may relate to CD14 upregulation on the surface of macrophages.
American Psychological Association (APA)
Zhou, Jianyun& Yan, Jun& Liang, Huaping& Jiang, Jianxin. 2014. Epinephrine Enhances the Response of Macrophages under LPS Stimulation. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016182
Modern Language Association (MLA)
Zhou, Jianyun…[et al.]. Epinephrine Enhances the Response of Macrophages under LPS Stimulation. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1016182
American Medical Association (AMA)
Zhou, Jianyun& Yan, Jun& Liang, Huaping& Jiang, Jianxin. Epinephrine Enhances the Response of Macrophages under LPS Stimulation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016182
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016182