Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis
Joint Authors
García, Maria de los Angeles
Borrero, Reinier
Lanio, Maria E.
Tirado, Yanely
Alvarez, Nadine
Puig, Alina
Aguilar, Alicia
Canet, Liem
Mata Espinoza, Dulce
Barrios Payán, Jorge
Sarmiento, María Elena
Acosta, Armando
Hernández-Pando, Rogelio
Norazmi, Mohd. Nor
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-07
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A more effective vaccine against tuberculosis (TB) is urgently needed.
Based on its high genetic homology with Mycobacterium tuberculosis (Mtb), the nonpathogenic mycobacteria, Mycobacterium smegmatis (Ms), could be an attractive source of potential antigens to be included in such a vaccine.
We evaluated the capability of lipid-based preparations obtained from Ms to provide a protective response in Balb/c mice after challenge with Mtb H37Rv strain.
The intratracheal model of progressive pulmonary TB was used to assess the level of protection in terms of bacterial load as well as the pathological changes in the lungs of immunized Balb/c mice following challenge with Mtb.
Mice immunized with the lipid-based preparation from Ms either adjuvanted with Alum (LMs-AL) or nonadjuvanted (LMs) showed significant reductions in bacterial load ( P < 0.01 ) compared to the negative control group (animals immunized with phosphate buffered saline (PBS)).
Both lipid formulations showed the same level of protection as Bacille Calmette and Guerin (BCG).
Regarding the pathologic changes in the lungs, mice immunized with both lipid formulations showed less pneumonic area when compared with the PBS group ( P < 0.01 ) and showed similar results compared with the BCG group.
These findings suggest the potential of LMs as a promising vaccine candidate against TB.
American Psychological Association (APA)
García, Maria de los Angeles& Borrero, Reinier& Lanio, Maria E.& Tirado, Yanely& Alvarez, Nadine& Puig, Alina…[et al.]. 2014. Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1016194
Modern Language Association (MLA)
García, Maria de los Angeles…[et al.]. Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1016194
American Medical Association (AMA)
García, Maria de los Angeles& Borrero, Reinier& Lanio, Maria E.& Tirado, Yanely& Alvarez, Nadine& Puig, Alina…[et al.]. Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1016194
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016194