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Functional Screening of Antibiotic Resistance Genes from a Representative Metagenomic Library of Food Fermenting Microbiota
Joint Authors
Devirgiliis, Chiara
Zinno, Paola
Stirpe, Mariarita
Barile, Simona
Perozzi, Giuditta
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Lactic acid bacteria (LAB) represent the predominant microbiota in fermented foods.
Foodborne LAB have received increasing attention as potential reservoir of antibiotic resistance (AR) determinants, which may be horizontally transferred to opportunistic pathogens.
We have previously reported isolation of AR LAB from the raw ingredients of a fermented cheese, while AR genes could be detected in the final, marketed product only by PCR amplification, thus pointing at the need for more sensitive microbial isolation techniques.
We turned therefore to construction of a metagenomic library containing microbial DNA extracted directly from the food matrix.
To maximize yield and purity and to ensure that genomic complexity of the library was representative of the original bacterial population, we defined a suitable protocol for total DNA extraction from cheese which can also be applied to other lipid-rich foods.
Functional library screening on different antibiotics allowed recovery of ampicillin and kanamycin resistant clones originating from Streptococcus salivarius subsp.
thermophilus and Lactobacillus helveticus genomes.
We report molecular characterization of the cloned inserts, which were fully sequenced and shown to confer AR phenotype to recipient bacteria.
We also show that metagenomics can be applied to food microbiota to identify underrepresented species carrying specific genes of interest.
American Psychological Association (APA)
Devirgiliis, Chiara& Zinno, Paola& Stirpe, Mariarita& Barile, Simona& Perozzi, Giuditta. 2014. Functional Screening of Antibiotic Resistance Genes from a Representative Metagenomic Library of Food Fermenting Microbiota. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1016206
Modern Language Association (MLA)
Devirgiliis, Chiara…[et al.]. Functional Screening of Antibiotic Resistance Genes from a Representative Metagenomic Library of Food Fermenting Microbiota. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1016206
American Medical Association (AMA)
Devirgiliis, Chiara& Zinno, Paola& Stirpe, Mariarita& Barile, Simona& Perozzi, Giuditta. Functional Screening of Antibiotic Resistance Genes from a Representative Metagenomic Library of Food Fermenting Microbiota. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1016206
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016206