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Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment
Joint Authors
Ibrahim, Evra Raunie
Hossain, Md. Anowar
Roslan, Hairul Azman
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Sago palm (Metroxylon sagu) is a perennial plant native to Southeast Asia and exploited mainly for the starch content in its trunk.
Genetic improvement of sago palm is extremely slow when compared to other annual starch crops.
Urgent attention is needed to improve the sago palm planting material and can be achieved through nonconventional methods.
We have previously developed a tissue culture method for sago palm, which is used to provide the planting materials and to develop a genetic transformation procedure.
Here, we report the genetic transformation of sago embryonic callus derived from suspension culture using Agrobacterium tumefaciens and gene gun systems.
The transformed embryoids cells were selected against Basta (concentration 10 to 30 mg/L).
Evidence of foreign genes integration and function of the bar and gus genes were verified via gene specific PCR amplification, gus staining, and dot blot analysis.
This study showed that the embryogenic callus was the most suitable material for transformation as compared to the fine callus, embryoid stage, and initiated shoots.
The gene gun transformation showed higher transformation efficiency than the ones transformed using Agrobacterium when targets were bombarded once or twice using 280 psi of helium pressure at 6 to 8 cm distance.
American Psychological Association (APA)
Ibrahim, Evra Raunie& Hossain, Md. Anowar& Roslan, Hairul Azman. 2014. Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1016239
Modern Language Association (MLA)
Ibrahim, Evra Raunie…[et al.]. Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1016239
American Medical Association (AMA)
Ibrahim, Evra Raunie& Hossain, Md. Anowar& Roslan, Hairul Azman. Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1016239
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016239