Differentially Expressed MicroRNAs in Maternal Plasma for the Noninvasive Prenatal Diagnosis of Down Syndrome (Trisomy 21)
Joint Authors
Kamhieh-Milz, Julian
Moftah, Reham Fadl Hassan
Bal, Gürkan
Futschik, Matthias
Sterzer, Viktor
Khorramshahi, Omid
Burow, Martin
Thiel, Gundula
Stuke-Sontheimer, Annegret
Chaoui, Rabih
Kamhieh-Milz, Sundrela
Salama, Abdulgabar
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
|Objectives.
Most developmental processes are under the control of small regulatory RNAs called microRNAs (miRNAs).
We hypothesize that different fetal developmental processes might be reflected by extracellular miRNAs in maternal plasma and may be utilized as biomarkers for the noninvasive prenatal diagnosis of chromosomal aneuploidies.
In this proof-of-concept study, we report on the identification of extracellular miRNAs in maternal plasma of Down syndrome (DS) pregnancies.
Methods.
Using high-throughput quantitative PCR (HT-qPCR), 1043 miRNAs were investigated in maternal plasma via comparison of seven DS pregnancies with age and fetal sex matched controls.
Results.
Six hundred and ninety-five miRNAs were identified.
Thirty-six significantly differentially expressed mature miRNAs were identified as potential biomarkers.
Hierarchical cluster analysis of these miRNAs resulted in the clear discrimination of DS from euploid pregnancies.
Gene targets of the differentially expressed miRNAs were enriched in signaling pathways such as mucin type-O-glycans, ECM-receptor interactions, TGF-beta, and endocytosis, which have been previously associated with DS.
Conclusions.
miRNAs are promising and stable biomarkers for a broad range of diseases and may allow a reliable, cost-efficient diagnostic tool for the noninvasive prenatal diagnosis of DS.
American Psychological Association (APA)
Kamhieh-Milz, Julian& Moftah, Reham Fadl Hassan& Bal, Gürkan& Futschik, Matthias& Sterzer, Viktor& Khorramshahi, Omid…[et al.]. 2014. Differentially Expressed MicroRNAs in Maternal Plasma for the Noninvasive Prenatal Diagnosis of Down Syndrome (Trisomy 21). BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1016271
Modern Language Association (MLA)
Kamhieh-Milz, Julian…[et al.]. Differentially Expressed MicroRNAs in Maternal Plasma for the Noninvasive Prenatal Diagnosis of Down Syndrome (Trisomy 21). BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1016271
American Medical Association (AMA)
Kamhieh-Milz, Julian& Moftah, Reham Fadl Hassan& Bal, Gürkan& Futschik, Matthias& Sterzer, Viktor& Khorramshahi, Omid…[et al.]. Differentially Expressed MicroRNAs in Maternal Plasma for the Noninvasive Prenatal Diagnosis of Down Syndrome (Trisomy 21). BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1016271
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016271