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Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes
Joint Authors
Zhang, Jianli
Guo, Fujun
Mi, Jianqiang
Zhang, Zhiye
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
|Objectives.
Temporomandibular joint (TMJ) disorders are common disease in maxillofacial surgery.
The aim of this study is to regenerate fibrocartilage with a mixture of TMJ fibrochondrocytes and periodontal ligament derived mesenchymal stem cells (PD-MSCs).
Materials and Methods.
Fibrochondrocytes and PD-MSC were cocultured (ratio 1 : 1) for 3 weeks.
Histology and glycosaminoglycans (GAGs) assay were performed to examine the deposition of GAG.
Green florescent protein (GFP) was used to track PD-MSC.
Conditioned medium of PD-MSCs was collected to study the soluble factors.
Gene expression of fibrochondrocytes cultured in conditioned medium was tested by quantitative PCR (qPCR).
Results.
Increased proliferation of TMJ-CH was observed in coculture pellets when compared to monoculture.
Enhanced GAG production in cocultures was shown by histology and GAG quantification.
Tracing of GFP revealed the fact that PD-MSC disappears after coculture with TMJ-CH for 3 weeks.
In addition, conditioned medium of PD-MSC was also shown to increase the proliferation and GAG deposition of TMJ-CH.
Meanwhile, results of qPCR demonstrated that conditioned medium enhanced the expression levels of matrix-related genes in TMJ-CH.
Conclusions.
Results from this study support the mechanism of MSC-chondrocyte interaction, in which MSCs act as secretor of soluble factors that stimulate proliferation and extracellular matrix deposition of chondrocytes.
American Psychological Association (APA)
Zhang, Jianli& Guo, Fujun& Mi, Jianqiang& Zhang, Zhiye. 2014. Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016276
Modern Language Association (MLA)
Zhang, Jianli…[et al.]. Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1016276
American Medical Association (AMA)
Zhang, Jianli& Guo, Fujun& Mi, Jianqiang& Zhang, Zhiye. Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016276
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016276