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A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus
Joint Authors
Wang, He-Xiang
Zhao, Shuang
Rong, Cheng-Bo
Kong, Chang
Liu, Yu
Xu, Feng
Miao, Qian-Jiang
Wang, Shou-Xian
Zhang, Guo-Qing
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A novel laccase was isolated and purified from fermentation mycelia of mushroom Coprinus comatus with an isolation procedure including three ion-exchange chromatography steps on DEAE-cellulose, CM-cellulose, and Q-Sepharose and one gel-filtration step by fast protein liquid chromatography on Superdex 75.
The purified enzyme was a monomeric protein with a molecular weight of 64 kDa.
It possessed a unique N-terminal amino acid sequence of AIGPVADLKV, which has considerably high sequence similarity with that of other fungal laccases, but is different from that of C.
comatus laccases reported.
The enzyme manifested an optimal pH value of 2.0 and an optimal temperature of 60°C using 2,2′-azinobis(3-ethylbenzothiazolone-6-sulfonic acid) diammonium salt (ABTS) as the substrate.
The laccase displayed, at pH 2.0 and 37°C, K m values of 1.59 mM towards ABTS.
It potently suppressed proliferation of tumor cell lines HepG2 and MCF7, and inhibited human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) with an IC50 value of 3.46 μM, 4.95 μM, and 5.85 μM, respectively, signifying that it is an antipathogenic protein.
American Psychological Association (APA)
Zhao, Shuang& Rong, Cheng-Bo& Kong, Chang& Liu, Yu& Xu, Feng& Miao, Qian-Jiang…[et al.]. 2014. A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016282
Modern Language Association (MLA)
Zhao, Shuang…[et al.]. A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1016282
American Medical Association (AMA)
Zhao, Shuang& Rong, Cheng-Bo& Kong, Chang& Liu, Yu& Xu, Feng& Miao, Qian-Jiang…[et al.]. A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016282
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016282