TolC Promotes ExPEC Biofilm Formation and Curli Production in Response to Medium Osmolarity
Joint Authors
Hou, Bo
Meng, Xian-Rong
Zhang, Li-Yuan
Tan, Chen
Jin, Hui
Zhou, Rui
Gao, Jian-Feng
Wu, Bin
Li, Zi-Li
Liu, Mei
Chen, Huan-Chun
Bi, Ding-Ren
Li, Shao-Wen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
While a high osmolarity medium activates Cpx signaling and causes CpxR to repress csgD expression, and efflux protein TolC protein plays an important role in biofilm formation in Escherichia coli, whether TolC also responds to an osmolarity change to regulate biofilm formation in extraintestinal pathogenic E.
coli (ExPEC) remains unknown.
In this study, we constructed ΔtolC mutant and complement ExPEC strains to investigate the role of TolC in the retention of biofilm formation and curli production capability under different osmotic conditions.
The ΔtolC mutant showed significantly decreased biofilm formation and lost the ability to produce curli fimbriae compared to its parent ExPEC strain PPECC42 when cultured in M9 medium or 1/2 M9 medium of increased osmolarity with NaCl or sucrose at 28°C.
However, biofilm formation and curli production levels were restored to wild-type levels in the ΔtolC mutant in 1/2 M9 medium.
We propose for the first time that TolC protein is able to form biofilm even under high osmotic stress.
Our findings reveal an interplay between the role of TolC in ExPEC biofilm formation and the osmolarity of the surrounding environment, thus providing guidance for the development of a treatment for ExPEC biofilm formation.
American Psychological Association (APA)
Hou, Bo& Meng, Xian-Rong& Zhang, Li-Yuan& Tan, Chen& Jin, Hui& Zhou, Rui…[et al.]. 2014. TolC Promotes ExPEC Biofilm Formation and Curli Production in Response to Medium Osmolarity. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1016373
Modern Language Association (MLA)
Hou, Bo…[et al.]. TolC Promotes ExPEC Biofilm Formation and Curli Production in Response to Medium Osmolarity. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1016373
American Medical Association (AMA)
Hou, Bo& Meng, Xian-Rong& Zhang, Li-Yuan& Tan, Chen& Jin, Hui& Zhou, Rui…[et al.]. TolC Promotes ExPEC Biofilm Formation and Curli Production in Response to Medium Osmolarity. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1016373
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016373