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Phenotypic Characterization of Mycoplasma synoviae Induced Changes in the Metabolic and Sensitivity Profile of In Vitro Infected Chicken Chondrocytes
Joint Authors
Dušanić, Daliborka
Benčina, Dušan
Narat, Mojca
Oven, Irena
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In infectious synovitis caused by Mycoplasma synoviae chicken chondrocytes (CCH) may come into direct contact with these bacteria that are also capable of invading CCH in vitro.
In this study, phenotype microarrays were used to evaluate the influence of Mycoplasma synoviae on the global metabolic activity of CCH.
Therefore, CCH were cultured in the presence of 504 individual compounds, spotted in wells of 11 phenotype microarrays for eukaryotic cells, and exposed to Mycoplasma synoviae membranes or viable Mycoplasma synoviae.
Metabolic activity and sensitivity of normal cells versus infected cells were evaluated.
Metabolic profiles of CCH treated with viable Mycoplasma synoviae or its membranes were significantly different from those of CCH alone.
CCH treated with Mycoplasma synoviae membranes were able to use 48 carbon/nitrogen sources not used by CCH alone.
Treatment also influenced ion uptake in CCH and intensified the sensitivity to 13 hormones, 5 immune mediators, and 29 cytotoxic chemicals.
CCH were even more sensitive to hormones/immune mediators when exposed to viable Mycoplasma synoviae.
Our results indicate that exposure to Mycoplasma synoviae or its membranes induces a wide range of metabolic and sensitivity modifications in CCH that can contribute to pathological processes in the development of infectious synovitis.
American Psychological Association (APA)
Dušanić, Daliborka& Benčina, Dušan& Narat, Mojca& Oven, Irena. 2014. Phenotypic Characterization of Mycoplasma synoviae Induced Changes in the Metabolic and Sensitivity Profile of In Vitro Infected Chicken Chondrocytes. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1016395
Modern Language Association (MLA)
Dušanić, Daliborka…[et al.]. Phenotypic Characterization of Mycoplasma synoviae Induced Changes in the Metabolic and Sensitivity Profile of In Vitro Infected Chicken Chondrocytes. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1016395
American Medical Association (AMA)
Dušanić, Daliborka& Benčina, Dušan& Narat, Mojca& Oven, Irena. Phenotypic Characterization of Mycoplasma synoviae Induced Changes in the Metabolic and Sensitivity Profile of In Vitro Infected Chicken Chondrocytes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1016395
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016395