A Time-Dose Model to Quantify the Antioxidant Responses of the Oxidative Hemolysis Inhibition Assay (OxHLIA) and Its Extension to Evaluate Other Hemolytic Effectors
Joint Authors
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-26
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The development of a convenient mathematical application for testing the antioxidant potential of standard and novel therapeutic agents is essential for the research community to perform evaluations in a more precise form.
The in vitro oxidative hemolysis inhibition assay, despite its relevance for in vivo responses, lacks a proper mathematical model to quantify the responses.
In this work, a simple nonlinear time-dose tool to test the effectiveness of antioxidant compounds is presented.
The model was verified with available experimental data from the bibliography.
The model helps to describe accurately the antioxidant response as a function of time and dose allowing comparisons between compounds.
Its advantages are a simple application, provision of parametric estimates that characterize the response, simplification of the protocol, economization of experimental effort, and facilitation of rigorous comparisons among the effects of different compounds and experimental approaches.
Finally, other effectors that may obstruct or be of interest for the antioxidant determination are also modeled in similar principles.
Thus, the basis of more complex multivariable models is provided.
In all experimental data fitted, the calculated parameters were always statistically significant, the equations prove to be consistent, and the correlation coefficient of determination was in all cases higher than 0.98.
American Psychological Association (APA)
Prieto, M. A.& Vázquez, J. A.. 2014. A Time-Dose Model to Quantify the Antioxidant Responses of the Oxidative Hemolysis Inhibition Assay (OxHLIA) and Its Extension to Evaluate Other Hemolytic Effectors. BioMed Research International،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1016412
Modern Language Association (MLA)
Prieto, M. A.& Vázquez, J. A.. A Time-Dose Model to Quantify the Antioxidant Responses of the Oxidative Hemolysis Inhibition Assay (OxHLIA) and Its Extension to Evaluate Other Hemolytic Effectors. BioMed Research International No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1016412
American Medical Association (AMA)
Prieto, M. A.& Vázquez, J. A.. A Time-Dose Model to Quantify the Antioxidant Responses of the Oxidative Hemolysis Inhibition Assay (OxHLIA) and Its Extension to Evaluate Other Hemolytic Effectors. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1016412
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016412