Dynamic Contact Angle Analysis of Protein Adsorption on Polysaccharide Multilayer’s Films for Biomaterial Reendothelialization
Joint Authors
Benni, Safiya
Avramoglou, Thierry
Hlawaty, Hanna
Mora, Laurence
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Atherosclerosis is a major cardiovascular disease.
One of the side effects is restenosis.
The aim of this work was to study the coating of stents by dextran derivates based polyelectrolyte’s multilayer (PEM) films in order to increase endothelialization of injured arterial wall after stent implantation.
Films were composed with diethylaminoethyl dextran (DEAE) as polycation and dextran sulphate (DS) as polyanion.
One film was composed with 4 bilayers of (DEAE-DS)4 and was labeled D−.
The other film was the same as D− but with an added terminal layer of DEAE polycation: (DEAE-DS)4-DEAE (labeled D+).
The dynamic adsorption/desorption of proteins on the films were characterized by dynamic contact angle (DCA) and atomic force microscopy (AFM).
Human endothelial cell (HUVEC) adhesion and proliferation were quantified and correlated to protein adsorption analyzed by DCA for fibronectin, vitronectin, and bovine serum albumin (BSA).
Our results showed that the endothelial cell response was optimal for films composed of DS as external layer.
Fibronectin was found to be the only protein to exhibit a reversible change in conformation after desorption test.
This behavior was only observed for (DEAE-DS)4 films.
(DEAE-DS)4 films could enhance HUVEC proliferation in agreement with fibronectin ability to easily change from conformation.
American Psychological Association (APA)
Benni, Safiya& Avramoglou, Thierry& Hlawaty, Hanna& Mora, Laurence. 2014. Dynamic Contact Angle Analysis of Protein Adsorption on Polysaccharide Multilayer’s Films for Biomaterial Reendothelialization. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1016444
Modern Language Association (MLA)
Benni, Safiya…[et al.]. Dynamic Contact Angle Analysis of Protein Adsorption on Polysaccharide Multilayer’s Films for Biomaterial Reendothelialization. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1016444
American Medical Association (AMA)
Benni, Safiya& Avramoglou, Thierry& Hlawaty, Hanna& Mora, Laurence. Dynamic Contact Angle Analysis of Protein Adsorption on Polysaccharide Multilayer’s Films for Biomaterial Reendothelialization. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1016444
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016444