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Cervical Cancer Cell Supernatants Induce a Phenotypic Switch from U937-Derived Macrophage-Activated M1 State into M2-Like Suppressor Phenotype with Change in Toll-Like Receptor Profile
Joint Authors
Sánchez-Reyes, Karina
Bravo-Cuellar, Alejandro
Hernández-Flores, Georgina
Lerma-Díaz, José Manuel
Jave-Suárez, Luis Felipe
Gómez-Lomelí, Paulina
de Celis, Ruth
Aguilar-Lemarroy, Adriana
Domínguez-Rodríguez, Jorge Ramiro
Ortiz-Lazareno, Pablo Cesar
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Cervical cancer (CC) is the second most common cancer among women worldwide.
Infection with human papillomavirus (HPV) is the main risk factor for developing CC.
Macrophages are important immune effector cells; they can be differentiated into two phenotypes, identified as M1 (classically activated) and M2 (alternatively activated).
Macrophage polarization exerts profound effects on the Toll-like receptor (TLR) profile.
In this study, we evaluated whether the supernatant of human CC cells HeLa, SiHa, and C-33A induces a shift of M1 macrophage toward M2 macrophage in U937-derived macrophages.
Results.
The results showed that soluble factors secreted by CC cells induce a change in the immunophenotype of macrophages from macrophage M1 into macrophage M2.
U937-derived macrophages M1 released proinflammatory cytokines and nitric oxide; however, when these cells were treated with the supernatant of CC cell lines, we observed a turnover of M1 toward M2.
These cells increased CD163 and IL-10 expression.
The expression of TLR-3, -7, and -9 is increased when the macrophages were treated with the supernatant of CC cells.
Conclusions.
Our result strongly suggests that CC cells may, through the secretion of soluble factors, induce a change of immunophenotype M1 into M2 macrophages.
American Psychological Association (APA)
Sánchez-Reyes, Karina& Bravo-Cuellar, Alejandro& Hernández-Flores, Georgina& Lerma-Díaz, José Manuel& Jave-Suárez, Luis Felipe& Gómez-Lomelí, Paulina…[et al.]. 2014. Cervical Cancer Cell Supernatants Induce a Phenotypic Switch from U937-Derived Macrophage-Activated M1 State into M2-Like Suppressor Phenotype with Change in Toll-Like Receptor Profile. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1016454
Modern Language Association (MLA)
Sánchez-Reyes, Karina…[et al.]. Cervical Cancer Cell Supernatants Induce a Phenotypic Switch from U937-Derived Macrophage-Activated M1 State into M2-Like Suppressor Phenotype with Change in Toll-Like Receptor Profile. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1016454
American Medical Association (AMA)
Sánchez-Reyes, Karina& Bravo-Cuellar, Alejandro& Hernández-Flores, Georgina& Lerma-Díaz, José Manuel& Jave-Suárez, Luis Felipe& Gómez-Lomelí, Paulina…[et al.]. Cervical Cancer Cell Supernatants Induce a Phenotypic Switch from U937-Derived Macrophage-Activated M1 State into M2-Like Suppressor Phenotype with Change in Toll-Like Receptor Profile. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1016454
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016454