Minocycline Enhances the Effectiveness of NociceptinOrphanin FQ during Neuropathic Pain
Joint Authors
Popiolek-Barczyk, Katarzyna
Rojewska, Ewelina
Jurga, Agnieszka M.
Makuch, Wioletta
Zador, Ferenz
Borsodi, Anna
Piotrowska, Anna
Przewlocka, Barbara
Mika, Joanna
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Nociceptin/orphanin FQ (N/OFQ) antinociception, which is mediated selectively by the N/OFQ peptide receptor (NOP), was demonstrated in pain models.
In this study, we determine the role of activated microglia on the analgesic effects of N/OFQ in a rat model of neuropathic pain induced by chronic constriction injury (CCI) to the sciatic nerve.
Repeated 7-day administration of minocycline (30 mg/kg i.p.), a drug that affects microglial activation, significantly reduced pain in CCI-exposed rats and it potentiates the analgesic effects of administered N/OFQ (2.5–5 μg i.t.).
Minocycline also downregulates the nerve injury-induced upregulation of NOP protein in the dorsal lumbar spinal cord.
Our in vitro study showed that minocycline reduced NOP mRNA, but not protein, level in rat primary microglial cell cultures.
In [35S]GTPγS binding assays we have shown that minocycline increases the spinal N/OFQ-stimulated NOP signaling.
We suggest that the modulation of the N/OFQ system by minocycline is due to the potentiation of its neuronal antinociceptive activity and weakening of the microglial cell activation.
This effect is beneficial for pain relief, and these results suggest new targets for the development of drugs that are effective against neuropathic pain.
American Psychological Association (APA)
Popiolek-Barczyk, Katarzyna& Rojewska, Ewelina& Jurga, Agnieszka M.& Makuch, Wioletta& Zador, Ferenz& Borsodi, Anna…[et al.]. 2014. Minocycline Enhances the Effectiveness of NociceptinOrphanin FQ during Neuropathic Pain. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1016545
Modern Language Association (MLA)
Popiolek-Barczyk, Katarzyna…[et al.]. Minocycline Enhances the Effectiveness of NociceptinOrphanin FQ during Neuropathic Pain. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1016545
American Medical Association (AMA)
Popiolek-Barczyk, Katarzyna& Rojewska, Ewelina& Jurga, Agnieszka M.& Makuch, Wioletta& Zador, Ferenz& Borsodi, Anna…[et al.]. Minocycline Enhances the Effectiveness of NociceptinOrphanin FQ during Neuropathic Pain. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1016545
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016545