Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR
Joint Authors
Cai, Yicun
Li, Xiang
Lv, Rong
Yang, Jielin
Li, Jian
He, Yuping
Pan, Liangwen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In this project, a highly precise quantitative method based on the digital polymerase chain reaction (dPCR) technique was developed to determine the weight of pork and chicken in meat products.
Real-time quantitative polymerase chain reaction (qPCR) is currently used for quantitative molecular analysis of the presence of species-specific DNAs in meat products.
However, it is limited in amplification efficiency and relies on standard curves based Ct values, detecting and quantifying low copy number target DNA, as in some complex mixture meat products.
By using the dPCR method, we find the relationships between the raw meat weight and DNA weight and between the DNA weight and DNA copy number were both close to linear.
This enabled us to establish formulae to calculate the raw meat weight based on the DNA copy number.
The accuracy and applicability of this method were tested and verified using samples of pork and chicken powder mixed in known proportions.
Quantitative analysis indicated that dPCR is highly precise in quantifying pork and chicken in meat products and therefore has the potential to be used in routine analysis by government regulators and quality control departments of commercial food and feed enterprises.
American Psychological Association (APA)
Cai, Yicun& Li, Xiang& Lv, Rong& Yang, Jielin& Li, Jian& He, Yuping…[et al.]. 2014. Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1016574
Modern Language Association (MLA)
Cai, Yicun…[et al.]. Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1016574
American Medical Association (AMA)
Cai, Yicun& Li, Xiang& Lv, Rong& Yang, Jielin& Li, Jian& He, Yuping…[et al.]. Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1016574
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016574