Lentivirus Mediated siRNA against GluN2B Subunit of NMDA Receptor Reduces Nociception in a Rat Model of Neuropathic Pain
Joint Authors
Wu, Feixiang
Pan, Ruirui
Chen, Jiaying
Sugita, Megumi
Chen, Caiyang
Tao, Yong
Yu, Weifeng
Sun, Yuming
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Although neuropathic pain (NP) is still not fully understood by scientists and clinicians alike, studies suggest that N-methyl-D-aspartate (NMDA) receptors play an important role in the induction and maintenance of NP.
A promising treatment for NP is through the downregulation of NMDA subunit GluN2B by RNA interference; however, naked siRNA (small interference RNA) is not effective in long-term treatments.
In order to concoct a viable prolonged treatment for NP, Lv-siGluN2B (lentivirus carrying siRNA targeting GluN2B subunit) was prepared and the antinociception effects were observed in chronic constriction injury (CCI) rats in the present study.
Results showed that Lv-siGluN2B was transduced into spinal cord cells after intrathecal injections and effectively reduced the nociception induced by sciatic nerve ligation while inhibiting the mRNA and protein expression of GluN2B.
This antinociception effect lasted approximately 7 weeks.
These findings suggest that GluN2B subunit could be a target for NP treatment and Lv-siGluN2B represents a new potential option for long-term treatment of NP.
American Psychological Association (APA)
Wu, Feixiang& Pan, Ruirui& Chen, Jiaying& Sugita, Megumi& Chen, Caiyang& Tao, Yong…[et al.]. 2014. Lentivirus Mediated siRNA against GluN2B Subunit of NMDA Receptor Reduces Nociception in a Rat Model of Neuropathic Pain. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1016614
Modern Language Association (MLA)
Wu, Feixiang…[et al.]. Lentivirus Mediated siRNA against GluN2B Subunit of NMDA Receptor Reduces Nociception in a Rat Model of Neuropathic Pain. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1016614
American Medical Association (AMA)
Wu, Feixiang& Pan, Ruirui& Chen, Jiaying& Sugita, Megumi& Chen, Caiyang& Tao, Yong…[et al.]. Lentivirus Mediated siRNA against GluN2B Subunit of NMDA Receptor Reduces Nociception in a Rat Model of Neuropathic Pain. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1016614
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016614