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Hyperuricemic PRP in Tendon Cells
Joint Authors
Rubio-Azpeitia, Eva
Andia, I.
Maffulli, Nicola
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Platelet-rich plasma (PRP) is injected within tendons to stimulate healing.
Metabolic alterations such as the metabolic syndrome, diabetes, or hyperuricemia could hinder the therapeutic effect of PRP.
We hypothesise that tendon cells sense high levels of uric acid and this could modify their response to PRP.
Tendon cells were treated with allogeneic PRPs for 96 hours.
Hyperuricemic PRP did not hinder the proliferative actions of PRP.
The gene expression pattern of inflammatory molecules in response to PRP showed absence of IL-1b and COX1 and modest expression of IL6, IL8, COX2, and TGF-b1.
IL8 and IL6 proteins were secreted by tendon cells treated with PRP.
The synthesis of IL6 and IL8 proteins induced by PRP is decreased significantly in the presence of hyperuricemia (P = 0.017 and P = 0.012, resp.).
Concerning extracellular matrix, PRP-treated tendon cells displayed high type-1 collagen, moderate type-3 collagen, decorin, and hyaluronan synthase-2 expression and modest expression of scleraxis.
Hyperuricemia modified the expression pattern of extracellular matrix proteins, upregulating COL1 (P = 0.036) and COMP (P = 0.012) and downregulating HAS2 (P = 0.012).
Positive correlations between TGF-b1 and type-1 collagen (R = 0.905, P = 0.002) and aggrecan (R = 0.833, P = 0.010) and negative correlations between TGF-b1 and IL6 synthesis (R = −0.857, P = 0.007) and COX2 (R = −0.810, P = 0.015) were found.
American Psychological Association (APA)
Andia, I.& Rubio-Azpeitia, Eva& Maffulli, Nicola. 2014. Hyperuricemic PRP in Tendon Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016645
Modern Language Association (MLA)
Andia, I.…[et al.]. Hyperuricemic PRP in Tendon Cells. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1016645
American Medical Association (AMA)
Andia, I.& Rubio-Azpeitia, Eva& Maffulli, Nicola. Hyperuricemic PRP in Tendon Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1016645
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016645