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Automated Image Analysis of Lung Branching Morphogenesis from Microscopic Images of Fetal Rat Explants
Joint Authors
Rodrigues, Pedro L.
Rodrigues, Nuno F.
Duque, Duarte
Granja, Sara
Correia-Pinto, Jorge
Vilaça, João L.
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Regulating mechanisms of branching morphogenesis of fetal lung rat explants have been an essential tool for molecular research.
This work presents a new methodology to accurately quantify the epithelial, outer contour, and peripheral airway buds of lung explants during cellular development from microscopic images.
Methods.
The outer contour was defined using an adaptive and multiscale threshold algorithm whose level was automatically calculated based on an entropy maximization criterion.
The inner lung epithelium was defined by a clustering procedure that groups small image regions according to the minimum description length principle and local statistical properties.
Finally, the number of peripheral buds was counted as the skeleton branched ends from a skeletonized image of the lung inner epithelia.
Results.
The time for lung branching morphometric analysis was reduced in 98% in contrast to the manual method.
Best results were obtained in the first two days of cellular development, with lesser standard deviations.
Nonsignificant differences were found between the automatic and manual results in all culture days.
Conclusions.
The proposed method introduces a series of advantages related to its intuitive use and accuracy, making the technique suitable to images with different lighting characteristics and allowing a reliable comparison between different researchers.
American Psychological Association (APA)
Rodrigues, Pedro L.& Rodrigues, Nuno F.& Duque, Duarte& Granja, Sara& Correia-Pinto, Jorge& Vilaça, João L.. 2014. Automated Image Analysis of Lung Branching Morphogenesis from Microscopic Images of Fetal Rat Explants. Computational and Mathematical Methods in Medicine،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1016834
Modern Language Association (MLA)
Rodrigues, Pedro L.…[et al.]. Automated Image Analysis of Lung Branching Morphogenesis from Microscopic Images of Fetal Rat Explants. Computational and Mathematical Methods in Medicine No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1016834
American Medical Association (AMA)
Rodrigues, Pedro L.& Rodrigues, Nuno F.& Duque, Duarte& Granja, Sara& Correia-Pinto, Jorge& Vilaça, João L.. Automated Image Analysis of Lung Branching Morphogenesis from Microscopic Images of Fetal Rat Explants. Computational and Mathematical Methods in Medicine. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1016834
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1016834