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Toona Sinensis and Moschus Decoction Induced Cell Cycle Arrest in Human Cervical Carcinoma HeLa Cells
Joint Authors
Zhen, Hong
Zhang, Yifei
Fang, Zhijia
Huang, Zhiwei
You, Chongge
Shi, Ping
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Toona sinensis and Moschus are two herb materials used in traditional Chinese medicine, most commonly for their various biological activities.
In this study, we investigated the inhibitory effect of three decoctions from Toona sinensis, Moschus, and Toona sinensis and Moschus in combination on cell growth in several normal and cancer cell lines by cell viability assay.
The results showed that the combined decoction exhibited the strongest anticancer effects, compared to two single decoctions.
The observations indicated that the combined decoction did not induce cell apoptosis and autophagy in HeLa cells by fluorescence microscopy.
Flow cytometry analysis revealed that the combined decoction arrested HeLa cell cycle progression in S-phase.
After the decoction incubation, among 41 cell cycle related genes, eight were reduced, while five were increased in mRNA levels by real-time PCR assay.
Western blotting showed that there were no apparent changes of protein levels of Cyclin E1, while P27 expression significantly declined and the levels of CDC7 and CDK7 obviously increased.
The data suggest that the RB pathway is partially responsible for the decoction-induced S-phase cell cycle arrest in HeLa cells.
Therefore, the combined decoction may have therapeutic potential as an anticancer formula for certain cancers.
American Psychological Association (APA)
Zhen, Hong& Zhang, Yifei& Fang, Zhijia& Huang, Zhiwei& You, Chongge& Shi, Ping. 2014. Toona Sinensis and Moschus Decoction Induced Cell Cycle Arrest in Human Cervical Carcinoma HeLa Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018099
Modern Language Association (MLA)
Zhen, Hong…[et al.]. Toona Sinensis and Moschus Decoction Induced Cell Cycle Arrest in Human Cervical Carcinoma HeLa Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1018099
American Medical Association (AMA)
Zhen, Hong& Zhang, Yifei& Fang, Zhijia& Huang, Zhiwei& You, Chongge& Shi, Ping. Toona Sinensis and Moschus Decoction Induced Cell Cycle Arrest in Human Cervical Carcinoma HeLa Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018099
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018099