Effect of Rat Medicated Serum Containing You Gui Wan on Mouse Oocyte In Vitro Maturation and Subsequent Fertilization Competence
Joint Authors
Jiang, Xiao-Hui
Deng, Yan-li
Lu, Hua
Duan, Heng
Zhen, Xia
Hu, Xiang
Liang, Xin
Yie, Shang-mian
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
You Gui Wan (YGW) is a classic herbal formula in traditional Chinese medicine (TCM) used for the clinical treatment of infertility.
This study was to explore whether YGW has an impact on mouse oocyte maturation in vitro and subsequent fertilization competence.
Rat medicated serum containing YGW was prepared by orally administrating YGW.
Mouse immature oocytes were cultured with YGW medicated serum and compared to those cultured with or without normal rat serum or follicle-stimulating hormone (FSH).
YGW medicated serum significantly increased the percentages of matured oocytes when compared to the groups with or without normal rat serum (P < 0.01).
Furthermore, YGW medicated serum increased the rate of in vitro fertilization (IVF) when compared to the groups treated with FSH and with or without normal rat serum (P < 0.001).
YGW medicated serum also had significant effects on the mRNA expressions of PKA, CREB, MAPK, PKC, PKG, and MPF and the concentrations of cAMP, cGMP, and NO in matured oocytes.
These results indicate that YGW can promote mouse oocyte maturation and IVF in vitro.
Signaling pathways, such as the cAMP/PKA/MAPK, the PKC-MAPK, and the NO-cGMP-PKG pathway, which are similar to those induced by FSH, may be responsible for this action.
American Psychological Association (APA)
Jiang, Xiao-Hui& Deng, Yan-li& Lu, Hua& Duan, Heng& Zhen, Xia& Hu, Xiang…[et al.]. 2014. Effect of Rat Medicated Serum Containing You Gui Wan on Mouse Oocyte In Vitro Maturation and Subsequent Fertilization Competence. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1018157
Modern Language Association (MLA)
Jiang, Xiao-Hui…[et al.]. Effect of Rat Medicated Serum Containing You Gui Wan on Mouse Oocyte In Vitro Maturation and Subsequent Fertilization Competence. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1018157
American Medical Association (AMA)
Jiang, Xiao-Hui& Deng, Yan-li& Lu, Hua& Duan, Heng& Zhen, Xia& Hu, Xiang…[et al.]. Effect of Rat Medicated Serum Containing You Gui Wan on Mouse Oocyte In Vitro Maturation and Subsequent Fertilization Competence. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1018157
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018157