Protective Effect of Danhong Injection on Acute Hepatic Failure Induced by Lipopolysaccharide and D-Galactosamine in Mice
Joint Authors
Wang, Ying
Gao, Li-Na
Cui, Yuan-Lu
Jiang, Heng-Li
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Acute hepatic failure (AHF), which leads to an extremely high mortality rate, has become the focus of attention in clinic.
In this study, Danhong injection (DHI) was investigated to evaluate the preventive and protective effect on AHF induced by lipopolysaccharide (LPS) and D-galactosamine (GalN) in mice.
For AHF induction, ICR mice were intraperitoneally injected with D-GalN (700 mg/kg) and LPS (20 μg/kg).
DHI was administrated twice, at 12 and 1 h, respectively, before D-GalN/LPS injection.
After stimulation with D-GalN/LPS for 1 and 6 h, serum and livers were collected for analysis.
We found that mice administrated with DHI displayed a higher survival rate, lower serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil), glutathione S-transferase (GST), and tumor necrosis factor (TNF)-α.
DHI inhibited the elevations of hepatic lipid peroxidation (malondialdehyde), caspase-8 activity, and mRNA expression levels of inflammatory cytokines (interleukin-1β and interleukin-6) increased by D-GalN/LPS in the liver.
Furthermore, liver histopathological analysis indicated that the DHI group showed markedly fewer apoptotic (TUNEL positive) cells and less pathological changes than those in the AHF model group.
These results provide a novel insight into the pharmacological actions of DHI as a potential candidate for treating AHF.
American Psychological Association (APA)
Wang, Ying& Gao, Li-Na& Cui, Yuan-Lu& Jiang, Heng-Li. 2014. Protective Effect of Danhong Injection on Acute Hepatic Failure Induced by Lipopolysaccharide and D-Galactosamine in Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018160
Modern Language Association (MLA)
Wang, Ying…[et al.]. Protective Effect of Danhong Injection on Acute Hepatic Failure Induced by Lipopolysaccharide and D-Galactosamine in Mice. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1018160
American Medical Association (AMA)
Wang, Ying& Gao, Li-Na& Cui, Yuan-Lu& Jiang, Heng-Li. Protective Effect of Danhong Injection on Acute Hepatic Failure Induced by Lipopolysaccharide and D-Galactosamine in Mice. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018160
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018160