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Persea declinata (Bl.) Kosterm Bark Crude Extract Induces Apoptosis in MCF-7 Cells via G0G1 Cell Cycle Arrest, Bcl-2BaxBcl-xl Signaling Pathways, and ROS Generation
Joint Authors
Narrima, Putri
Paydar, Mohammadjavad
Looi, Chung Yeng
Wong, Yi Li
Taha, Hairin
Wong, Won Fen
Ali Mohd, Mustafa
Hadi, A. Hamid A.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-07
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Persea declinata (Bl.) Kosterm is a member of the Lauraceae family, widely distributed in Southeast Asia.
It is from the same genus with avocado (Persea americana Mill), which is widely consumed as food and for medicinal purposes.
In the present study, we examined the anticancer properties of Persea declinata (Bl.) Kosterm bark methanolic crude extract (PDM).
PDM exhibited a potent antiproliferative effect in MCF-7 human breast cancer cells, with an IC50 value of 16.68 µg/mL after 48 h of treatment.
We observed that PDM caused cell cycle arrest and subsequent apoptosis in MCF-7 cells, as exhibited by increased population at G0/G1 phase, higher lactate dehydrogenase (LDH) release, and DNA fragmentation.
Mechanistic studies showed that PDM caused significant elevation in ROS production, leading to perturbation of mitochondrial membrane potential, cell permeability, and activation of caspases-3/7.
On the other hand, real-time PCR and Western blot analysis showed that PDM treatment increased the expression of the proapoptotic molecule, Bax, but decreased the expression of prosurvival proteins, Bcl-2 and Bcl-xL, in a dose-dependent manner.
These findings imply that PDM could inhibit proliferation in MCF-7 cells via cell cycle arrest and apoptosis induction, indicating its potential as a therapeutic agent worthy of further development.
American Psychological Association (APA)
Narrima, Putri& Paydar, Mohammadjavad& Looi, Chung Yeng& Wong, Yi Li& Taha, Hairin& Wong, Won Fen…[et al.]. 2014. Persea declinata (Bl.) Kosterm Bark Crude Extract Induces Apoptosis in MCF-7 Cells via G0G1 Cell Cycle Arrest, Bcl-2BaxBcl-xl Signaling Pathways, and ROS Generation. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1018270
Modern Language Association (MLA)
Narrima, Putri…[et al.]. Persea declinata (Bl.) Kosterm Bark Crude Extract Induces Apoptosis in MCF-7 Cells via G0G1 Cell Cycle Arrest, Bcl-2BaxBcl-xl Signaling Pathways, and ROS Generation. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-1018270
American Medical Association (AMA)
Narrima, Putri& Paydar, Mohammadjavad& Looi, Chung Yeng& Wong, Yi Li& Taha, Hairin& Wong, Won Fen…[et al.]. Persea declinata (Bl.) Kosterm Bark Crude Extract Induces Apoptosis in MCF-7 Cells via G0G1 Cell Cycle Arrest, Bcl-2BaxBcl-xl Signaling Pathways, and ROS Generation. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-1018270
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018270