Inhibitory and Acceleratory Effects of Inonotus obliquus on Tyrosinase Activity and Melanin Formation in B16 Melanoma Cells
Joint Authors
Yan, Zheng-Fei
Yang, Yang
Tian, Feng-Hua
Mao, Xin-Xin
Li, Yu
Li, Chang-Tian
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The aim of the present study is to preliminarily investigate the antimelanogenesis effect of Inonotus obliquus extracts by cell-free mushroom tyrosinase assay.
It was found that petroleum ether and n-butanol extracts might contain unknown potential tyrosinase inhibitors, while its ethyl acetate extract might contain some unknown accelerators.
Six compounds were isolated and their structures were identified by interpretation of NMR data and nicotinic acid was first discovered in Inonotus obliquus.
In cells testing, betulin and trametenolic acid decreased tyrosinase activity and melanin content, while inotodiol and lanosterol significantly increased tyrosinase activity and melanin content, showing an AC50 of 9.74 and 8.43 μM, respectively.
Nicotinie acid, 3β,22,25-trihydroxy-lanosta-8-ene, had a little or no effect on tyrosinase.
Betulin exhibited a mode of noncompetitive inhibition with a KI=KIS of 0.4 μM on tyrosinase activity showing an IC50 of 5.13 μM and being more effective than kojic acid (6.43 μM), and trametenolic acid exhibited a mode of mixed inhibition with a KI of 0.9 μM, KIS of 0.5 μM, and an IC50 of 7.25 μM.
We proposed betulin and trametenolic acid as a new candidate of potent tyrosinase inhibitors and inotodiol and lanosterol as accelerators that could be used as therapeutic agent.
American Psychological Association (APA)
Yan, Zheng-Fei& Yang, Yang& Tian, Feng-Hua& Mao, Xin-Xin& Li, Yu& Li, Chang-Tian. 2014. Inhibitory and Acceleratory Effects of Inonotus obliquus on Tyrosinase Activity and Melanin Formation in B16 Melanoma Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1018304
Modern Language Association (MLA)
Yan, Zheng-Fei…[et al.]. Inhibitory and Acceleratory Effects of Inonotus obliquus on Tyrosinase Activity and Melanin Formation in B16 Melanoma Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1018304
American Medical Association (AMA)
Yan, Zheng-Fei& Yang, Yang& Tian, Feng-Hua& Mao, Xin-Xin& Li, Yu& Li, Chang-Tian. Inhibitory and Acceleratory Effects of Inonotus obliquus on Tyrosinase Activity and Melanin Formation in B16 Melanoma Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1018304
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018304