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Protection of Tong-Sai-Mai Decoction against Apoptosis Induced by H2O2 in PC12 Cells: Mechanisms via Bcl-2-Mitochondria-ROS-INOS Pathway
Joint Authors
Lee, Maxwell Kim Kit
Lu, Yin
Di, Liu-qing
Xu, Hui-qin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-22
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Tong-Sai-Mai decoction (TSM) is a Chinese materia medica polyherbal formulation that has been applied in treating brain ischemia for hundreds of years.
Because it could repress the oxidative stress in in vivo studies, now we focus on the in vitro studies to investigate the mechanism by targeting the oxidative stress dependent signaling.
The relation between the neurogenesis and the reactive oxygen species (ROS) production remains largely unexamined.
PC12 cells are excitable cell types widely used as in vitro model for neuronal cells.
Most marker genes that are related to neurotoxicity, apoptosis, and cell cycles are expressed at high levels in these cells.
The aim of the present study is to explore the cytoprotection of TSM against hydrogen peroxide- (H2O2-) induced apoptosis and the molecular mechanisms underlying PC12 cells.
Our findings revealed that TSM cotreatment with H2O2 restores the expression of bcl-2, inducible nitric oxide synthase (INOS), and mitochondria membrane potential.
Meanwhile, it reduces intracellular [Ca2+] concentration, lactate dehydrogenase (LDH) release, and the expression of caspase-3 and bax.
The results of the present study suggested that the cytoprotective effects of the TSM might be mediated, at least in part, by the bcl-2-mitochondria-ROS-INOS pathway.
Due to its nontoxic characteristics, TSM could be further developed to treat the neurodegenerative diseases which are closely associated with the oxidative stress.
American Psychological Association (APA)
Lee, Maxwell Kim Kit& Lu, Yin& Di, Liu-qing& Xu, Hui-qin. 2014. Protection of Tong-Sai-Mai Decoction against Apoptosis Induced by H2O2 in PC12 Cells: Mechanisms via Bcl-2-Mitochondria-ROS-INOS Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-18.
https://search.emarefa.net/detail/BIM-1018400
Modern Language Association (MLA)
Lee, Maxwell Kim Kit…[et al.]. Protection of Tong-Sai-Mai Decoction against Apoptosis Induced by H2O2 in PC12 Cells: Mechanisms via Bcl-2-Mitochondria-ROS-INOS Pathway. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-18.
https://search.emarefa.net/detail/BIM-1018400
American Medical Association (AMA)
Lee, Maxwell Kim Kit& Lu, Yin& Di, Liu-qing& Xu, Hui-qin. Protection of Tong-Sai-Mai Decoction against Apoptosis Induced by H2O2 in PC12 Cells: Mechanisms via Bcl-2-Mitochondria-ROS-INOS Pathway. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-18.
https://search.emarefa.net/detail/BIM-1018400
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018400