Generation of the Bovine Viral Diarrhea Virus E0 Protein in Transgenic Astragalus and Its Immunogenicity in Sika Deer
Joint Authors
Gao, Yugang
Zhao, Xueliang
Zang, Pu
Liu, Qun
Wei, Gongqing
Zhang, Lianxue
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The bovine viral diarrhea virus (BVDV), a single-stranded RNA virus, can cause fatal diarrhea syndrome, respiratory problems, and reproductive disorders in herds.
Over the past few years, it has become clear that the BVDV infection rates are increasing and it is likely that an effective vaccine for BVDV will be needed.
In this study, transgenic Astragalus was used as an alternative productive platform for the expression of glycoprotein E0.
The immunogenicity of glycoprotein E0 expressed in transgenic Astragalus was detected in deer.
The presence of pBI121-E0 was confirmed by polymerase chain reaction (PCR), transcription was verified by reverse transcription- (RT-) PCR, and recombinant protein expression was confirmed by ELISA and Western blot analyses.
Deer that were immunized subcutaneously with the transgenic plant vaccine developed specific humoral and cell-mediated immune responses against BVDV.
This study provides a new method for a protein with weak immunogenicity to be used as part of a transgenic plant vaccine.
American Psychological Association (APA)
Gao, Yugang& Zhao, Xueliang& Zang, Pu& Liu, Qun& Wei, Gongqing& Zhang, Lianxue. 2014. Generation of the Bovine Viral Diarrhea Virus E0 Protein in Transgenic Astragalus and Its Immunogenicity in Sika Deer. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1018406
Modern Language Association (MLA)
Gao, Yugang…[et al.]. Generation of the Bovine Viral Diarrhea Virus E0 Protein in Transgenic Astragalus and Its Immunogenicity in Sika Deer. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1018406
American Medical Association (AMA)
Gao, Yugang& Zhao, Xueliang& Zang, Pu& Liu, Qun& Wei, Gongqing& Zhang, Lianxue. Generation of the Bovine Viral Diarrhea Virus E0 Protein in Transgenic Astragalus and Its Immunogenicity in Sika Deer. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1018406
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018406