Guanxinkang Decoction Exerts Its Antiatherosclerotic Effect Partly through Inhibiting the Endoplasmic Reticulum Stress
Joint Authors
Wang, Hao
Zheng, Zhi-Min
Gao, Bu-Lang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Purpose.
To investigate the antiatherosclerotic effect of Guanxinkang (GXK) decoction on the apoptosis, mitochondrial membrane potential (MMP), and endoplasmic reticulum stress (ERS) of human umbilical vein endothelial cells (HUVEC) pretreated with homocysteinemia (HCY).
Materials and Methods.
HUVEC were randomly divided into 5 groups: (1) blank control group (control), (2) model control group (model), (3) GXK low dose group, (4) GXK medium dose group, and (5) GXK high dose group.
For the three GXK groups, HCY was given to reach the concentration of 3.0 mmol/L after HUVEC had been incubated with rabbit serum containing GXK for two hours.
At 3, 6, 12, and 24 h after HCY had been incubated with the cells, the HUVEC were collected for test of the apoptosis rate, MMP, and GRP78 protein (reflecting ERS).
Results.
In the model control group, the apoptosis rate and GRP 78 protein expression of HUVEC significantly increased ( P < 0.05 ), while MMP significantly decreased ( P < 0.05 ) compared with the blank control group.
After GXK treatment of medium and high doses, the apoptosis rate and the GRP 78 protein expression significantly ( P < 0.05 ) decreased, while MMP significantly increased ( P < 0.05 ) in a time-dependent manner compared with the model control group.
Conclusion.
GXK can antagonize the injury of HUVEC caused by HCY and the antagonism effect increases with the concentration and treatment duration of GXK, with the possible mechanism of GXK antagonism being through inhibiting ERS caused by HCY.
American Psychological Association (APA)
Wang, Hao& Zheng, Zhi-Min& Gao, Bu-Lang. 2014. Guanxinkang Decoction Exerts Its Antiatherosclerotic Effect Partly through Inhibiting the Endoplasmic Reticulum Stress. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018521
Modern Language Association (MLA)
Wang, Hao…[et al.]. Guanxinkang Decoction Exerts Its Antiatherosclerotic Effect Partly through Inhibiting the Endoplasmic Reticulum Stress. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1018521
American Medical Association (AMA)
Wang, Hao& Zheng, Zhi-Min& Gao, Bu-Lang. Guanxinkang Decoction Exerts Its Antiatherosclerotic Effect Partly through Inhibiting the Endoplasmic Reticulum Stress. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018521
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018521