Molecular detection and phylogenetic analysis of cutaneous fibro papillomatosis in Iraqi buffalo

Abstract EN

Bovine papillomatosis (BP) is conveyed worldwide among cattle, however, is moderately less regular in water buffalo.

The point of the present work was Molecular detection of cutaneous fibro papillomatosis in Buffalo in Al-Qadisiyah province, Iraq.

Forty cutaneous warts (CW) samples were collected from lesions distributed on any part of the body (shoulder, thigh, around the eye region, and in the perineal region) of buffaloes in different ages.

Out of 40 samples subjected to PCR, 26 samples were positive for BPVs.

From the 26 positive samples, 4 samples were sent for DNA sequence analyses and found two samples was positive for Deltapapillomavirus (BPV-1) and two samples positive for Xipapillomavirus (BPV-4).

The sequencing analysis for the BPV L1 fragment was identified from the CW that shown a 100% identity with BPV-4 from Iraq (KY662042.1_1) and BPV-1 sequences from Poland (KX594402.1 and KF284141.1).

The molecular and phylogenetic results of the current study reported the similarity and close relation between the BPV-1 and 4 genotypes of bovine and buffalo in Iraqi breeds and other BPV strains from other countries.

is conveyed worldwide among cattle, however, is moderately less regular in water buffalo.

The point of the present work was Molecular detection of cutaneous fibro papillomatosis in Buffalo in Al-Qadisiyah province, Iraq.

Forty cutaneous warts (CW) samples were collected from lesions distributed on any part of the body (shoulder, thigh, around the eye region, and in the perineal region) of buffaloes in different ages.

Out of 40 samples subjected to PCR, 26 samples were positive for BPVs.

From the 26 positive samples, 4 samples were sent for DNA sequence analyses and found two samples was positive for Deltapapillomavirus (BPV-1) and two samples positive for Xipapillomavirus (BPV-4).

The sequencing analysis for the BPV L1 fragment was identified from the CW that shown a 100% identity with BPV-4 from Iraq (KY662042.1_1) and BPV-1 sequences from Poland (KX594402.1 and KF284141.1).

The molecular and phylogenetic results of the current study reported the similarity and close relation between the BPV-1 and 4 genotypes of bovine and buffalo in Iraqi breeds and other BPV strains from other countries.