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Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells
Joint Authors
Gao, Chao
Cui, Yu-Gui
Sun, Dalin
Wang, Zhiqiang
Jin, Baofang
Zhang, Xindong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
To investigate the effect of Yangjing Capsule (YC) extract on proliferation of GC-1 spermatogonia (spg) cells and the mechanism.
Methods.
GC-1 spg cells were treated with 0.01, 0.1, and 1 mg/mL YC extract.
MTT assay was performed to detect the cell viability.
Flow cytometry was used to measure the cell cycle and apoptosis of GC-1 spg cells.
Real-time PCR and western blot were applied to determine the mRNA and protein expression of Oct-4 and Plzf.
Gfrα1 knockdown and LY294002 (PI3K inhibitor) were applied to explore the underlying mechanism.
Results.
After 48 h treatment of YC, the viability of GC-1 spg cells increased significantly and the ratio of apoptotic cells reduced significantly.
The increased mRNA and protein expression of Oct-4 and Plzf suggested YC promoted self-renewal of GC-1 spg cells.
Both Gfrα1 siRNAs and LY294002 treatments held back YC extract’s stimulation effects on mRNA and protein expression of Oct-4 and Plzf and consequently inhibited the proliferation of GC-1 spg cells induced by YC extract.
Conclusion.
YC extract could stimulate the proliferation of GC-1 spg cells.
Partly via Gfrα1, YC extract is able to trigger the activation of PI3K pathway and finally lead to self-renewal of GC-1 spg cells.
American Psychological Association (APA)
Wang, Zhiqiang& Jin, Baofang& Zhang, Xindong& Cui, Yu-Gui& Sun, Dalin& Gao, Chao. 2014. Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1018839
Modern Language Association (MLA)
Wang, Zhiqiang…[et al.]. Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1018839
American Medical Association (AMA)
Wang, Zhiqiang& Jin, Baofang& Zhang, Xindong& Cui, Yu-Gui& Sun, Dalin& Gao, Chao. Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1018839
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018839