Phenolic Profiling and Evaluation of Contraceptive Effect of the Ethanolic Extract of Salsola imbricata Forssk. in Male Albino Rats
Joint Authors
Shehab, Naglaa Gamil
Abu-Gharbieh, Eman
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Reported researches dealing with either composition or bioactivity of Salsola imbricata are limited.
This study was conducted aiming to investigate the phenolic composition of the plant and evaluate its efficacy as male contraceptive.
Polyphenols, namely, phenolic acids and flavonoids, were qualitatively and quantitatively analysed by RP-HPLC in the hydrolysed methanol extract using two different wavelengths, 280 and 330 nm.
The efficiency of different solvents in extracting the plant phenolics was assessed via spectrophotometric determination of the total phenolic and flavonoid contents.
Acute toxicity study was carried out on the ethanolic extract to ascertain its safety prior to biological evaluation.
The contraceptive effect was assessed, in male rats, by oral administration of the extract at two doses (250 and 500 mg/kg b.
wt.), over a period of 65 days.
HPLC analyses allowed the identification and quantification of a total of 13 and 8 components in the hydrolysed-methanol extract; the overall phenolic composition was dominated by quercitrin (12.692%) followed by coumaric acid (4.251%).
Prolonged oral administration of the ethanolic extract caused slight reduction in the testis weight only.
A significant decrease in the sperm count was observed (P<0.01) in the two treated groups while significant decrease in the epididymal sperm motility was only observed in the high dose group.
Morphological abnormalities were observed in sperms of treated animals.
No distinct change in serum FSH, LH, and testosterone concentration was recorded.
The histopathological findings supported to a high extent these results.
The male contraceptive activity of Salsola imbricata could be ascribed to its phenolic components, especially quercitrin.
American Psychological Association (APA)
Shehab, Naglaa Gamil& Abu-Gharbieh, Eman. 2014. Phenolic Profiling and Evaluation of Contraceptive Effect of the Ethanolic Extract of Salsola imbricata Forssk. in Male Albino Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018895
Modern Language Association (MLA)
Shehab, Naglaa Gamil& Abu-Gharbieh, Eman. Phenolic Profiling and Evaluation of Contraceptive Effect of the Ethanolic Extract of Salsola imbricata Forssk. in Male Albino Rats. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1018895
American Medical Association (AMA)
Shehab, Naglaa Gamil& Abu-Gharbieh, Eman. Phenolic Profiling and Evaluation of Contraceptive Effect of the Ethanolic Extract of Salsola imbricata Forssk. in Male Albino Rats. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1018895
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018895