Da-Bu-Yin-Wan and Qian-Zheng-San to Neuroprotect the Mouse Model of Parkinson’s Disease
Joint Authors
Gong, Xiao-Gang
Sun, Hong-Mei
Zhang, Yi
Zhang, Shu-Jing
Gao, Yu-Shan
Feng, Jing
Hu, Jing-Hong
Gai, Cong
Guo, Zhen-Yu
Xu, Hong
Ma, Ling
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Da-Bu-Yin-Wan (DBYW) and Qian-Zheng-San (QZS), two classic traditional Chinese medicinal formulas, were clinically employed to treat Parkinson’s disease (PD).
Our previous studies demonstrated neuroprotective effects of them on mitochondrial function in PD mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).
The purpose of this research was to investigate their possible mechanisms in the light of mitochondrial ATP-sensitive potassium (mitoK ATP ) channels.
The neuroprotective effect of DBYW and QZS on dopamine (DA) neurons in substantia nigra (SN) in the MPTP-induced PD mice was investigated by behavioral test (pole test) and immunohistochemistry.
Adenosine triphosphate (ATP) level in the midbrain tissue was detected by firefly luciferase method.
MitoK ATP channel subunits SUR1 and Kir6.2 mRNA and protein expressions were tested by real-time PCR (RT-PCR) and Western blot.
It was observed that DBYW and/or QZS served to ameliorate MPTP-induced behavioral impairment and prevent the loss of substantia nigra dopamine neurons, as well as increase ATP level in the midbrain tissue and downregulate SUR1 expression at mRNA and protein levels with no marked influence on Kir6.2.
We concluded that DBYW and QZS exhibit neuroprotective effects probably through the regulation of ATP level and mitoK ATP channel subunit expressions.
American Psychological Association (APA)
Gong, Xiao-Gang& Sun, Hong-Mei& Zhang, Yi& Zhang, Shu-Jing& Gao, Yu-Shan& Feng, Jing…[et al.]. 2014. Da-Bu-Yin-Wan and Qian-Zheng-San to Neuroprotect the Mouse Model of Parkinson’s Disease. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1018975
Modern Language Association (MLA)
Gong, Xiao-Gang…[et al.]. Da-Bu-Yin-Wan and Qian-Zheng-San to Neuroprotect the Mouse Model of Parkinson’s Disease. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1018975
American Medical Association (AMA)
Gong, Xiao-Gang& Sun, Hong-Mei& Zhang, Yi& Zhang, Shu-Jing& Gao, Yu-Shan& Feng, Jing…[et al.]. Da-Bu-Yin-Wan and Qian-Zheng-San to Neuroprotect the Mouse Model of Parkinson’s Disease. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1018975
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018975