Extracts from Curcuma zedoaria Inhibit Proliferation of Human Breast Cancer Cell MDA-MB-231 In Vitro
Joint Authors
Gao, Xiu-fei
Li, Qing-lin
Li, Hai-long
Zhang, Hong-yan
Su, Jian-ying
Wang, Bei
Liu, Pei
Zhang, Ai-qin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
To evaluate the effect of petroleum ether extracts of Curcuma zedoaria on the proliferation of human triple negative breast cancer cell line MDA-MB-231.
Methods.
The reagents were isolated from Curcuma zedoaria by petroleum ether fraction.
It was assayed by CCK8 for MDA-MB-231 cellular viability with various concentrations and days, cell cycle analyses, Western Blot analysis, and Realtime Reverse Transcriptase PCR analyses for chemokines molecules including E-cadherin, and E-selectin, and adhesion molecules including CCR7, SLC, SDF-1, and CXCR4.
Epirubicin was used as control in the study.
Results.
MDA-MB-231 cells were inhibited by petroleum ether extracts of Curcuma zedoaria (P < 0.05), and the inhibition rate was dependent on concentrations and time.
Petroleum ether extracts of Curcuma zedoaria as well as Epirubicin produce a significant G0/G1 cell cycle arrest.
The level of expression of proteins E-cadherin and E-cadherin mRNA was significantly increased, while proteins SDF-1, CCR7, and CXCR4 mRNA were decreased after being incubated with petroleum ether extracts of Curcuma zedoaria at the concentrations of 300 μg/mL than control (P < 0.05).
The differences were that the protein CXCR4 mRNA expression level was higher than vehicle.
Conclusions.
MDA-MB-231 cells were inhibited by petroleum ether extracts of Curcuma zedoaria.
American Psychological Association (APA)
Gao, Xiu-fei& Li, Qing-lin& Li, Hai-long& Zhang, Hong-yan& Su, Jian-ying& Wang, Bei…[et al.]. 2014. Extracts from Curcuma zedoaria Inhibit Proliferation of Human Breast Cancer Cell MDA-MB-231 In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1018980
Modern Language Association (MLA)
Gao, Xiu-fei…[et al.]. Extracts from Curcuma zedoaria Inhibit Proliferation of Human Breast Cancer Cell MDA-MB-231 In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1018980
American Medical Association (AMA)
Gao, Xiu-fei& Li, Qing-lin& Li, Hai-long& Zhang, Hong-yan& Su, Jian-ying& Wang, Bei…[et al.]. Extracts from Curcuma zedoaria Inhibit Proliferation of Human Breast Cancer Cell MDA-MB-231 In Vitro. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1018980
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1018980