![](/images/graphics-bg.png)
Deciphering Molecular Mechanism Underlying Hypolipidemic Activity of Echinocystic Acid
Joint Authors
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Our previous study showed that a triterpene mixture, consisting of echinocystic acid (EA) and oleanolic acid (OA) at a ratio of 4 : 1, dose-dependently ameliorated the hyperlipidemia and atherosclerosis in rabbits fed with high fat/high cholesterol diets.
This study was aimed at exploring the mechanisms underlying antihyperlipidemic effect of EA.
Molecular docking simulation of EA was performed using Molegro Virtual Docker (version: 4.3.0) to investigate the potential targets related to lipid metabolism.
Based on the molecular docking information, isotope labeling method or spectrophotometry was applied to examine the effect of EA on the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, acyl-CoA:cholesterol acyltransferase (ACAT), and diacylglycerol acyltransferase (DGAT) in rat liver microsomes.
Our results revealed a strong affinity of EA towards ACAT and DGAT in molecular docking analysis, while low binding affinity existed between EA and HMG-CoA reductase as well as between EA and cholesteryl ester transfer protein.
Consistent with the results of molecular docking, in vitro enzyme activity assays showed that EA inhibited ACAT and DGAT, with IC50 values of 103 and 139 μM, respectively, and exhibited no significant effect on HMG-CoA reductase activity.
The present findings suggest that EA may exert hypolipidemic effect by inhibiting the activity of ACAT and DGAT.
American Psychological Association (APA)
Han, Li& Lai, Peng& Du, Jun-Rong. 2014. Deciphering Molecular Mechanism Underlying Hypolipidemic Activity of Echinocystic Acid. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1020292
Modern Language Association (MLA)
Han, Li…[et al.]. Deciphering Molecular Mechanism Underlying Hypolipidemic Activity of Echinocystic Acid. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1020292
American Medical Association (AMA)
Han, Li& Lai, Peng& Du, Jun-Rong. Deciphering Molecular Mechanism Underlying Hypolipidemic Activity of Echinocystic Acid. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1020292
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1020292