Protective Effect of Tea Polyphenol Ophthalmic Gel on Lens Epithelial Cells in Rabbits with Silicone Oil Tamponade after Vitrectomy
Joint Authors
Ma, Xianzhen
Guo, Dadong
Bi, Hongsheng
Xie, Xiaofeng
Guo, Junguo
Cui, Yan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Purpose.
The aim of this study was to investigate the effect of tea polyphenols (TP) ophthalmic gel on lens epithelial cells (LECs) in rabbits with silicone oil tamponade after vitrectomy.
Methods.
In this study, unilateral vitrectomy with silicone oil tamponade was performed using 2-month-old New Zealand white rabbits (n = 72); meanwhile, age-matched nonoperated rabbits (n = 18) were used as controls.
The TP ophthalmic gel was administered topically in the surgical eyes till they were sacrificed.
On days 45 and 90 after operation, the levels of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), and apoptosis of LECs were analyzed, respectively.
Meanwhile, caspase-3 mRNA and protein levels were also determined.
Results.
The results indicate that the levels of ROS and apoptosis were elevated for LECs in rabbits after operation, whereas ΔΨm was decreased.
Caspase-3 was apparently increased at both mRNA and protein levels.
Treatment of TP ophthalmic gel could reduce the generation of ROS, maintain ΔΨm, inhibit the overexpression of caspase-3, and thus decrease the apoptosis of LECs of rabbits after operation.
Conclusions.
TP ophthalmic gel can efficiently inhibit caspase-3 overexpression, reduce the apoptosis of LECs, and prevent LECs from damage.
Our result provides a new approach to prevent the development of complicated cataract after vitrectomy.
American Psychological Association (APA)
Ma, Xianzhen& Guo, Dadong& Bi, Hongsheng& Xie, Xiaofeng& Guo, Junguo& Cui, Yan. 2014. Protective Effect of Tea Polyphenol Ophthalmic Gel on Lens Epithelial Cells in Rabbits with Silicone Oil Tamponade after Vitrectomy. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1020300
Modern Language Association (MLA)
Ma, Xianzhen…[et al.]. Protective Effect of Tea Polyphenol Ophthalmic Gel on Lens Epithelial Cells in Rabbits with Silicone Oil Tamponade after Vitrectomy. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1020300
American Medical Association (AMA)
Ma, Xianzhen& Guo, Dadong& Bi, Hongsheng& Xie, Xiaofeng& Guo, Junguo& Cui, Yan. Protective Effect of Tea Polyphenol Ophthalmic Gel on Lens Epithelial Cells in Rabbits with Silicone Oil Tamponade after Vitrectomy. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1020300
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1020300