Effect of hibiscus sabdariffa and ceratonia silique aqueous extracts on the reproductive hormones of diabetic male rats

Abstract EN

The effects of aqueous extract of Hibiscus sabdariffa calyx and Ceratonia silique on feed intake, feed efficiency ratio, body weight gains, lipid profile, blood glucose, serum fertility hormones in diabetic rats were investigated.

Thirty adult male Sprague-Dawley rats were randomly divided into 6 equal groups.

Group (1) was fed on basal diet (negative control), while the other rats were fed on basal diet then rendered diabetic by intraperitoneal injection of alloxan (120 mg/kg/day) for 3 days.

Thereafter, group (2) was kept diabetic (positive control) and the other 4 groups were orally given 2 ml from condensed and diluted Hibiscus sabdariffa calyx and Ceratonia silique aqueous extracts which provided each rat with active component resulted from 100 mg and 200 mg/kg respectively.

Blood samples were collected for separating the serum which used for biochemical analyses.

Tests and pancreas specimens were taken for estimating histopathology.

The high active component found in concentrated Hibiscus sabdariffa calyx extract.

The results showed that oral administration of Hibiscus sabdariffa calyx and Ceratonia silique aqueous extracts to diabetic rats non-significant in feed intake, feed efficiency ratio, body weight gains and decreased serum levels of total cholesterol (TC), triglycerides (TG), low density lipoproteins (LDL-c), very low density lipoproteins (VLDL-c), prolactin and increased in high density lipoproteins (HDL-c).

Blood glucose decreased by administration of Hibiscus sabdariffa calyx and Ceratonia silique aqueous extracts.

It also increased in fertility hormones serum Testosterone, Folicle Stimulating Hormone, Luteinizing Hormone in diabetic rats.

Through these results and histopathology examinations illustrate the positive impact of both Hibiscus sabdariffa calyx and Ceratonia silique aqueous extracts in tests and pancreas.

These results point to the potential possibility of use Hibiscus sabdariffa calyx and Ceratonia silique aqueous extracts for the treatment of infertility diabetic patients.