Influence of thyme and leek on immunological functions and some haematological parameters in rats with cirrhotic liver

Abstract EN

This study aimed to determine the effect of dried thyme and dried leek supplementation on immune functions and some hematological parameters in cirrhotic liver rats.

Thirty-six rats weighing 170 ± 10 g were used for this purpose.

The first main group, a negative control (n=4) fed on the basal diet only.

The second main group (n =32) was injected intraperitoneal once with CCl₄ at a dose of 0.5ml/100 g body weight for the first time, after that a dose of 0.3 ml/100 g body weight was injected twice a week for six weeks to induce liver cirrhosis.

To ensure that livers become cirrhotic four rats were randomly chosen for pathological examination.

The rest of rats (n=28) were divided into (7) subgroups (4 rats each) as follows: Sub G1 (positive control) was fed on basal diet.

Sub G (2 and 3) were fed on a basal diet supplemented with 6% and 12 % dried thyme respectively.

Sub G (4 and 5) were supplemented with 6% and 12% dried leek respectively.

Sub G (6 and 7) were supplemented with 6% and 12 % of dried (thyme and leek) at a ratio of (1: 1) respectively.

Rats were fed on the experimental diet for eight weeks.

Results protected that the highest (IgG) and (IgM) levels in serum were noticed in the group fed on a combination of dried thyme and dried leek compared to positive control.

Diets supplemented with dried thyme, leek and their mixtures at different levels significantly increased (P<0.05) the levels of serum total protein, globulin and albumin compared to the control positive group.

Also, a significant decrease (P<0.05) in the levels of (AST), (ALP) and (ALT) were noticed in the groups fed on mixture of dried thyme and dried leek.

On the other hand, the supplementation with dried thyme, dried leek and their mixtures significantly (P<0.05) increased the mean level of WBCs, RBCs, PCV and Hemoglobin compared to the positive control.

The highest improvment in the tested parameters was observe in the groups fed on a mixture of dried thyme and dried leek at 12% .

It can be conclude that supplementation with dried thyme, dried leek, and their combination, could protect the liver cells from CCL4-induced liver damages.

The highest improvement was observed in the groups supplemented with, a combination of dried thyme and leek at a ratio of (1: 1) respectively.